The transcription of <it>MGAT4A </it>glycosyl transferase is increased in white cells of peripheral blood of Type 2 Diabetes patients

<p>Abstract</p> <p>Background</p> <p>Human glycosylase IV is involved in GLUT2 transporter regulation in pancreatic β cells. A KO of this gene along with a high fat diet in a mice model has been associated with the development of type 2 diabetes (T2D). The aims of this study were to measure and compare the <it>MGAT4A </it>mRNA levels in white blood cells (WBC) from T2D subjects and healthy subjects (T2NB), and to measure the half-life of the <it>MGAT4A </it>mRNA.</p> <p>Results</p> <p>We studied a sample of 73 individuals, 40 T2D subjects and 33 T2NB subjects. Anthropometrical and biochemical profiles were registered. The <it>MGAT4A </it>mRNA levels in WBC and the transcript half-life in Jurkat T cells were determined by Real-Time PCR. A blood differential cell counting was made for each individual. Cell counting showed T2D subjects exhibited an increased number of WBC compared to T2NB subjects (<it>P </it>= 0.0001). Biochemical parameters such as fasting glucose (<it>P </it>= 0.0001), and triglycerides (<it>P </it>= 0.002) were statistically significant. T2D subjects had 4.2-fold more <it>MGAT4A </it>transcript compared to T2NB subjects (<it>P </it>= 0.002). The <it>MGAT4A </it>mRNA had a half-life of 2.04 h in Jurkat T cells.</p> <p>Conclusion</p> <p>The results of this work suggest that in T2D subjects, high levels of glucose and triglycerides are accompanied by an increase on <it>MGAT4A </it>mRNA levels and WBC count; condition that suggests a pro-inflammatory state due to a chronic metabolic stress.</p>

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