Synthesis and characterization of hydroxyapatite from bovine bone for production of dental implants

This paper presents a study on the synthesis and characterization of hydroxyapatite that can be used to produce dental implants or serves as a replacement for other body hard tissues. The bioceramic material (hydroxyapatite) was derived from bovine bone samples after they were de-fatted and processed to produce particles measuring less than 250 μm. Processed bone powders were calcinated at 750℃ to obtain calcium phosphate compound (hydroxyapatite). This indicated complete elimination of the organic phase of the bone. The synthesized hydroxyapatite was physico-chemically characterized using Fourier transform infrared spectroscopy (FTIR), an optical microscope and scanning electron microscopy (SEM). Hydroxyapatite powder was sintered into a block and then characterized mechanically by measuring its hardness using a Rockwell hardness testing machine. The hydrophilicity and hydrophobicity of the sintered blocked specimens were studied. The average wettability of the substrate, which was a characteristic of the contact angle, was found to be approximately 31.73 degrees. This contact angle value is a measure of the material hydrophilicity. The biostability/corrosive resistance of the sintered samples was simulated in vitro in phosphate buffered saline solution (pH 7.4) by incubating in an optical incubator shaker (set at 60 revolutions per minute) to mimic physiological conditions. The results showed that hydroxyapatite can be extracted economically from a natural source such as bovine bone and can be employed as a restorative biomaterial for dental implants and hard tissue replacement.

Abstract licence: CC BY-NC

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