Adhesion to Vitronectin and Collagen I Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells

Abstract

The mechanisms controlling human mesenchymal stem cells (hMSC) differentiation are not entirely understood. We hypothesized that the contact with extracellular matrix (ECM) proteins normally found in bone marrow would promote osteogenic differentiation of hMSC in vitro. To test this hypothesis, we cultured hMSC on purified ECM proteins in the presence or absence of soluble osteogenic supplements, and assayed for the presence of well‐established differentiation markers (production of mineralized matrix, osteopontin, osteocalcin, collagen I, and alkaline phosphatase expression) over a 16‐day time course. We found that hMSC adhere to ECM proteins with varying affinity (fibronectin > collagen I ≥ collagen IV ≥ vitronectin > laminin‐1) and through distinct integrin receptors. Importantly, the greatest osteogenic differentiation occurred in cells plated on vitronectin and collagen I and almost no differentiation took place on fibronectin or uncoated plates. We conclude that the contact with vitronectin and collagen I promotes the osteogenic differentiation of hMSC, and that ECM contact alone may be sufficient to induce differentiation in these cells.

Cited by papers we hold 4

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• Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells · 2015
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• Nanotechnology in the regulation of stem cell behavior · 2013
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• Cell adhesion and mechanical stimulation in the regulation of mesenchymal stem cell differentiation · 2013
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