Leukotriene C4 biosynthesis in isolated August rat peritoneal leukocytes

The mixed leukocyte population obtained from the peritoneum of the August rat is a potentially important experimental model of inherent eosinophilia that has not been well characterized. In the present study, isolated cell preparations generated a concentration‐dependent release of leukotriene (LT) C 4 when exposed to the Ca 2+ ionophore A23187, reaching maximal stimulation at 5.0 μM. This response was inhibited by the 5‐lipoxygenase activating protein antagonist MK‐886 (0.1 μM), nominally Ca 2+ and Mg 2+ ‐free incubation media and by activation of protein kinase C via phorbol 12‐myristate 13‐acetate (50 nM). These findings establish a model system for investigating LTC 4 profiles contingent with innate peritoneal eosinophilia and are consistent with the hypothesis that cellular LTC 4 biosynthesis is phosphoregulated.

Abstract licence: CC BY

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