Protection of SH-SY5Y Neuronal Cells from Glutamate-Induced Apoptosis by 3, 6 ′ -Disinapoyl Sucrose, a Bioactive Compound Isolated from Radix Polygala

Abstract

The neuroprotective effects of 3, 6 ′ -disinapoyl sucrose (DISS) from Radix Polygala against glutamate-induced SH-SY5Y neuronal cells injury were evaluated in the present study. SH-SY5Y neuronal cells were pretreated with glutamate (8 mM) for 30 min followed by cotreatment with DISS for 12 h. Cell viability was determined by (3,4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT) assay, and apoptosis was confirmed by cell morphology and flow cytometry assay, evaluated with propidium iodide dye. Treatment with DISS (0.6, 6, and 60 μmol/L) increased cell viability dose dependently, inhibited LDH release, and attenuated apoptosis. The mechanisms by which DISS protected neuron cells from glutamate-induced excitotoxicity included the downregulation of proapoptotic gene Bax and the upregulation of antiapoptotic gene Bcl-2. The present findings indicated that DISS exerts neuroprotective effects against glutamate toxicity, which might be of importance and contribute to its clinical efficacy for the treatment of neurodegenerative diseases.

Cited by papers we hold 5

• Comprehensive Quality Assessment of Kaixin Powder by HPLC–DAD Quantification and HPLC–QTOF-MS/MS Confirmation · 2021
  Not checked
• Management of recurrent aphthous ulcers exploiting polymer-based Muco-adhesive sponges: in-vitro and in-vivo evaluation · 2021
  Not checked
• Sex-Specific Linkages Between Taxonomic and Functional Profiles of Tick Gut Microbiomes · 2019
  Not checked
• Researching glutamate – induced cytotoxicity in different cell lines: a comparative/collective analysis/study · 2015
  No notice
• Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds · 2014
  No notice

Reads

A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.