A Novel Image Cytometric Method for Quantitation of Immunohistochemical Staining of Cytoplasmic Antigens
M. Guillaud, J. B. Matthews, A. Harrison and 2 more
Analytical Cellular Pathology · 1997
doi:10.1155/1997/457297 (opens in a new tab)
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Abstract
Evaluation of molecular markers by immunohistochemical labelling of tissue sections has traditionally been performed by qualitative assessment by trained pathologists. For those markers with a staining component present outside of the nucleus, there has been no image histometric method available to reliably and consistently define cell interfaces within the tissue. We present a new method of approximating cellular boundaries to define cellular regions within which quantitative measurements of staining intensity may be made. The method is based upon Voronoi tessellation of a defined region of interest (ROI), and requires only the position of the nuclear centroids within the ROI.
Abstract licence: CC BY
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