Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012
Atsushi Yamanaka, Mami Matsuda, Tamaki Okabayashi and 9 more
mSphere · 2021
doi:10.1128/msphere.00339-21 (opens in a new tab)
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Abstract
Neutralization tests are the most reliable assay for flavivirus antibody detection; however, these assays are not suitable for high-throughput processing due to their time-consuming and labor-intensive nature. In this study, we developed single-round infectious particles (SRIPs) with a luciferase gene for dengue virus types 1 to 4, Japanese encephalitis virus, and Zika virus for use in a safe, high-throughput neutralization assay.
Abstract licence: CC BY
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