Skip to content
naulon

No retraction, correction or concern found when last checked on Oct 31, 2026.

Canola Proteins for Human Consumption: Extraction, Profile, and Functional Properties

Siong H. Tan, Rodney J. Mailer, Christopher L. Blanchard and 1 more

Journal of Food Science · 2010 · Review

doi:10.1111/j.1750-3841.2010.01930.x

Abstract

Canola protein isolate has been suggested as an alternative to other proteins for human food use due to a balanced amino acid profile and potential functional properties such as emulsifying, foaming, and gelling abilities. This is, therefore, a review of the studies on the utilization of canola protein in human food, comprising the extraction processes for protein isolates and fractions, the molecular character of the extracted proteins, as well as their food functional properties. A majority of studies were based on proteins extracted from the meal using alkaline solution, presumably due to its high nitrogen yield, followed by those utilizing salt extraction combined with ultrafiltration. Characteristics of canola and its predecessor rapeseed protein fractions such as nitrogen yield, molecular weight profile, isoelectric point, solubility, and thermal properties have been reported and were found to be largely related to the extraction methods. However, very little research has been carried out on the hydrophobicity and structure profiles of the protein extracts that are highly relevant to a proper understanding of food functional properties. Alkaline extracts were generally not very suitable as functional ingredients and contradictory results about many of the measured properties of canola proteins, especially their emulsification tendencies, have also been documented. Further research into improved extraction methods is recommended, as is a more systematic approach to the measurement of desired food functional properties for valid comparison between studies.

Shown for reading. Its publisher allows quoting it, not republishing it.

References 122

  1. 1.

    Adler-Nissen J, Olsen SH, Pour-El A. The influence of peptide chain length on taste and functional properties of enzymatically modified soy protein. Functionality and protein structure. 1979;125-46.

    Not in our library

  2. 2.

    Agboola SO, Ee KY, Mallon L, Zhao J. Isolation, characterization, and emulsifying properties of wattle seed (Acacia victoriae Bentham) extracts. J Agric Food Chem. 2007;55(14):5858-63.

    Not in our library10.1021/jf070656c

  3. 3.

    Aluko RE, McIntosh T. Polypeptide profile and functional properties of defatted meals and protein isolates of canola seeds. J Sci Food Agric. 2001;81(4):391-6.

    Not in our library

  4. 4.

    Aluko RE, McIntosh T. Electrophoretic and functional properties of mustard seed meals and protein concentrates. J Am Oil Chem Soc. 2004;81(7):679-83.

    Not in our library

  5. 5.

    Aluko RE, McIntosh T. Limited enzymatic proteolysis increases the level of incorporation of canola proteins into mayonnaise. Innov Food Sci Emerg Technol. 2005;6(2):195-202.

    Not in our library

  6. 6.

    Aluko RE, McIntosh T, Katepa-Mupondwa F. Comparative study of the polypeptide profiles and functional properties of Sinapis alba and Brassica juncea seed meals and protein concentrates. J Sci Food Agric. 2005;85(11):1931-7.

    Not in our library

  7. 7.

    Arntfield SD, Murray ED. The influence of processing parameters on food protein functionality. I. Differential scanning calorimetry as an indicator of protein denaturation. Can Inst Food Sci Technol. 1981;14(4):289.

    Not in our library

  8. 8.

    Australian oilseeds industry. Delivering high quality products to local and global markets [Internet]. 2009.

    Not in our library

  9. 9.

    Bell JM, Kimber DS, McGregor DI. Meal and by-product utilization in animal nutrition. Brassica oilseeds: production and utilization. 1995;301-37.

    Not in our library

  10. 10.

    Betschart AA, Fong RY, Saunders RM. Rice by-products: comparative extraction and precipitation of nitrogen from U.S. and Spanish bran and germ. J Food Sci. 1977;42(4):1088-93.

    Not in our library

  11. 11.

    Bhatty RS, McKenzie SL, Finlayson AJ. The proteins of rapeseed (Brassica napus L.) soluble in salt solutions. Biochem Cell Biol. 1968;46(10):1191-7.

    Not in our library10.1139/o68-178

  12. 12.

    Burgess DA. Purification, characterization and the micelle response of the 12S canola globulin. 1991;197.

    Not in our library

  13. 13.

    Cai R, Arntfield S. Thermal gelation in relation to binding of bovine serum albumin-polysaccharide systems. J Food Sci. 1997;62(6):1129-34.

    Not in our library

  14. 14.

    Campbell LD, Eggum BO, Jacobsen I. Biological value, amino acid availability and true metabolizable energy of low-glucosinolate rapeseed meal (canola) determined with rats and/or roosters. 1981.

    Not in our library

  15. 15.

    Canola oil and meal: standards and regulations. 1990;4.

    Not in our library

  16. 16.

    Canadian Canola Industry. Overview of Canada's Canola Industry [Internet]. 2009.

    Not in our library

  17. 17.

    Chung MWY, Lei B, Li-Chan ECY. Isolation and structural characterization of the major protein fraction from NorMan flaxseed (Linum usitatissimum L.). Food Chem. 2005;90(1–2):271-9.

    Not in our library

  18. 18.

    Czarnecki SD, Kritchevsky D, Liepa GU, Bietz DC, Beynen AC, Gorman MA. Dietary protein and atherosclerosis. Dietary proteins: how they alleviate disease and promote better health. 1992;42-56.

    Not in our library

  19. 19.

    Dalgalarrondo M, Robin JM, Azanza JL. Subunit composition of the globulin fraction of rapeseed (Brassica napus L.). Plant Sci. 1986;43(2):115-24.

    Not in our library

  20. 20.

    Damodaran S, Kinsella JE, Sousie WG. Interrelationship of molecular and functional properties of food proteins. Food proteins. 1989;21-51.

    Not in our library

  21. 21.

    Damodaran S, Damodaran S, Paraf A. Food proteins: an overview. Food proteins and their applications. 1997;1-21.

    Not in our library

  22. 22.

    Delisle J, Amiot J, Goulet G, Simard C, Brisson GJ, Jones JD. Nutritive value of protein fractions extracted from soybean, rapeseed and wheat flours in the rat. Plant Foods Hum Nutr. 1984;34(4):243-51.

    Not in our library

  23. 23.

    Diosady LL, Rubin LJ, Chen BK. Membrane based processes for the production of rapeseed protein isolates. 1989.

    Not in our library

  24. 24.

    Diosady LL, Tzeng YM, Rubin LJ. Preparation of rapeseed protein concentrates and isolates using ultrafiltration. J Food Sci. 1984;49(3):768-70.

    Not in our library

  25. 25.

    Downey RK, Bell JM, Shahidi F. New developments in canola research. Canola and rapeseed: production, chemistry, nutrition and processing technology. 1990;37-46.

    Not in our library

  26. 26.

    El-Adawy TA. Functional properties and nutritional quality of acetylated and succinylated mung bean protein isolate. Food Chemistry. 2000;70(1):83-91.

    Not in our library

  27. 27.

    El-Adawy TA, Rahma EH, El-Bedawey AA, Gafar AF. Nutritional potential and functional properties of sweet and bitter lupin seed protein isolates. Food Chem. 2001;74(4):455-62.

    Not in our library

  28. 28.

    El Nockrashy AS, Mukherjee KD, Mangold HK. Rapeseed protein isolates by countercurrent extraction and isoelectric precipitation. J Agric Food Chem. 1977;25(1):193-7.

    Not in our library10.1021/jf60209a022

  29. 29.

    Energy and protein requirements. 1985.

    Not in our library

  30. 30.

    Fischer W, Schopfer P. Isolation and characterization of mustard (Sinapis alba L.) seed storage proteins. Botanica Acta. 1988;101:48-56.

    Not in our library

  31. 31.

    Gao L, Nguyen KD, Utioh AC. Pilot scale recovery of proteins from a pea whey discharge by ultrafiltration. Lebensm Wiss Technol. 2001;34(3):149-58.

    Not in our library

  32. 32.

    Ghodsvali A, Khodaparast MHH, Vosoughi M, Diosady LL. Preparation of canola protein materials using membrane technology and evaluation of meals functional properties. Food Res Int. 2005;38(2):223-31.

    Not in our library

  33. 33.

    Gill TA, Tung MA. Thermally induced gelation of the 12S rapeseed glycoprotein. J Food Sci. 1978;43(5):1481-5.

    Not in our library

  34. 34.

    Halling PJ. Protein-stabilized foams and emulsions. Crit Rev Food Sci Nutri. 1981;15(2):155.

    Not in our library10.1080/10408398109527315

  35. 35.

    Husken A, Baumert A, Milkowski C, Becker HC, Strack D, Mollers C. Resveratrol glucoside (Piceid) synthesis in seeds of transgenic oilseed rape (Brassica napus L.). Theor Appl Genet. 2005;111(8):1553-62.

    Not in our library10.1007/s00122-005-0085-1

  36. 36.

    Hyun EH, Kang YJ. Gelation of rapeseed protein with microbial transglutaminase. Korean J Food Sci Technol. 1999;31(3):1262-7.

    Not in our library

  37. 37.

    Ismail F, Vaisey-Genser M, Fyfe B. Bitterness and astringency of sinapine and Its Components. J Food Sci. 1981;46(4):1241-4.

    Not in our library

  38. 38.

    Ismond MAH, Welsh WD. Application of new methodology to canola protein isolation. Food Chem. 1992;45(2):125-7.

    Not in our library

  39. 39.

    Jensen SK, Liu YG, Eggum BO. The effect of heat treatment on glucosinolates and nutritional value of rapeseed meal in rats. Anim Feed Sci Technol. 1995;53(1):17-28.

    Not in our library

  40. 40.

    Jensen SK, Olsen HS, Sorensen H, Shahidi F. Aqueous enzymatic processing of rapeseed for production of high quality products. Canola and rapeseed: production, chemistry, nutrition, and processing technology. 1990;331-43.

    Not in our library

All 122 references

Cited by papers we hold 1

Reads

Read by people on this page

None yet.

Checked or read by AI through naulon

None yet.

A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.

Are you an author?

Is this your paper?

Claim it to add your note and be paid when agents cite it. Free.