Three new RelE‐homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses

Abstract

Summary Prokaryotic toxin – antitoxin (TA) loci encode mRNA interferases that inhibit translation, either by cleaving mRNA codons at the ribosomal A site or by cleaving any RNA site‐specifically. So far, seven mRNA interferases of Escherichia coli have been identified, four of which cleave mRNA by a translation‐dependent mechanism. Here, we experimentally confirmed the presence of three novel TA loci in E. coli . We found that the yafNO, higBA (ygjNM) and ygiUT loci encode mRNA interferases related to RelE. YafO and HigB cleaved translated mRNA only, while YgiU cleaved RNA site‐specifically at GC[A/U], independently of translation. Thus, YgiU is the first RelE‐related mRNA interferase that cleaves mRNA independently of translation, in vivo . All three loci were induced by amino acid starvation, and inhibition of translation although to different degrees. Carbon starvation induced only two of the loci. The yafNO locus was induced by DNA damage, but the transcription originated from the dinB promoter. Thus, our results showed that the different TA loci responded differentially to environmental stresses. Induction of the three loci depended on Lon protease that may sense the environmental stresses and activate TA loci by cleavage of the antitoxins. Transcription of the three TA operons was autoregulated by the antitoxins.

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Cited by papers we hold 8

• ATP-Dependent Persister Formation in Escherichia coli · 2017
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• Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence · 2017
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• Toxin–antitoxin systems and their role in disseminating and maintaining antimicrobial resistance · 2017
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• Keeping the Wolves at Bay: Antitoxins of Prokaryotic Type II Toxin-Antitoxin Systems · 2016
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• Toxin-Antitoxin Systems as Multilevel Interaction Systems · 2014
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• Multiple Toxin-Antitoxin Systems in Mycobacterium tuberculosis · 2014
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• Characterization of a chromosomal type II toxin-antitoxin system mazEaFa in the Cyanobacterium Anabaena sp. PCC 7120. · 2013
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• Toxin-Antitoxin Systems Are Important for Niche-Specific Colonization and Stress Resistance of Uropathogenic Escherichia coli · 2012
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