Expression of insulin pathway genes during the period of caste determination in the honey bee, Apis mellifera

Abstract

Female honeybees have two castes, queens and workers. Developmental fate is determined by larval diet. Coding sequences made available through the Honey Bee Genome Sequencing Consortium allow for a pathway‐based approach to understanding caste determination. We examined the expression of several genes of the insulin signalling pathway, which is central to regulation of growth based on nutrition. We found one insulin‐like peptide expressed at very high levels in queen but not worker larvae. Also, the gene for an insulin receptor was expressed at higher levels in queen larvae during the 2nd larval instar. These results demonstrate that the insulin pathway is a compelling candidate for pursing the relationship between diet and downstream signals involved in caste determination and differentiation.

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References 27

1. 1.

   Britton JS, Lockwood WK, Li L, Cohen SM, Edgar BA. Drosophila's insulin/PI3-kinase pathway coordinates cellular metabolism with nutritional conditions. Dev Cell. 2002;2:239-249.

   Not in our library10.1016/s1534-5807(02)00117-x

2. 2.

   Dietz A, Hermann HR, Blum MS. The role of exogenous JH I, JH III and anti JH on queen induction of 4.5 day old worker honey bee larvae. J Insect Physiol. 1979;25:503-512.

   Not in our library

3. 3.

   Dogra GS, Ulrich GM, Rembold H. A comparative study of the endocrine system of the honey bee larvae under normal and experimental conditions. Z Naturforsch. 1977;32c:637-642.

   Not in our library

4. 4.
   Expression profiles during honeybee caste determination · 2001

   Not checked
5. 5.

   Evans JD, Wheeler DE. Gene expression and the evolution of insect polyphenisms. Bioessays. 2001;23:62-68.

   Not in our library10.1002/1521-1878(200101)23:1<62::aid-bies1008>3.0.co;2-7

6. 6.

   Glasscock JM, Mizoguchi A, Rachinsky A. Immunocytochemical localization of an allatotropin in developmental stages of Heliothis virescens and Apis mellifera. J Insect Physiol. 2005;51:345-355.

   Not in our library10.1016/j.jinsphys.2004.12.005

7. 7.

   Hepperle C, Hartfelder K. Differentially expressed regulatory genes in honey bee caste development. Naturwissenschaften. 2001;88:113-116.

   Not in our library10.1007/s001140000196

8. 8.

   Huber F. New Observations on Bees. 1821.

   Not in our library

9. 9.

   Ikeya T, Galic M, Belawat P, Nairz K, Hafen E. Nutrient-dependent expression of insulin-like peptides from neuroendocrine cells in the CNS contributes to growth regulation in Drosophila. Current Biol. 2002;12:1293-1300.

   Not in our library10.1016/s0960-9822(02)01043-6

10. 10.

    Laidlaw HH, Graham JJ. Production of queens and package bees. The Hive and the Honey Bee. 1992;989-1042.

    Not in our library

11. 11.

    Michener CD. The Social Behavior of the Bees. 1974.

    Not in our library

12. 12.

    Nijhout HF. The control of growth. Development. 2003;130:5863-5867.

    Not in our library10.1242/dev.00902

13. 13.

    O’Connor K, Baxter D. The demonstration of insulin-like material in the honey bee Apis mellifera. Comparative Biochem Physiol B. 1985;81:755-760.

    Not in our library

14. 14.

    Oldham S, Stocker H, Laffargue M, Wittwer F, Wymann M, Hafen E. The Drosophila insulin/IGF receptor controls growth and size by modulating PrdInsP3 levels. Development. 2002;129:4103-4109.

    Not in our library10.1242/dev.129.17.4103

15. 15.

    Rachinsky A. Brain and suboesophageal ganglion extracts affect juvenile hormone biosynthesis in honey bee larvae (Apis mellifera carnica). Zoology-Anal Complex Systems. 1996;99:277-284.

    Not in our library

16. 16.

    Rachinsky A, Feldlaufer MF. Responsiveness of honey bee (Apis mellifera) corpora allata to allatoregulatory peptides from four species. J Insect Physiol. 2000;46:41-46.

    Not in our library10.1016/s0022-1910(99)00100-6

17. 17.

    Rachinsky A, Strambi C, Strambi A, Hartfelder K. Caste and metamorphosis – hemolymph titers of juvenile hormone and ecdysteroids in last instar honeybee larvae. General Comparative Endocrinol. 1990;79:31-38.

    Not in our library10.1016/0016-6480(90)90085-z

18. 18.

    Rachinsky A, Tobe SS, Feldlaufer MF. Terminal steps in JH biosynthesis in the honey bee (Apis mellifera): developmental changes in sensitivity to JH precursor and allatotropin. Insect Biochem Mol Biol. 2000;30:729-737.

    Not in our library10.1016/s0965-1748(00)00044-8

19. 19.

    Rembold H, Czoppelt C, Grüne M, Pfeffer J, Woker E, Mauchamp B, Couillaud F, Baehr JC. Juvenile Hormone Titers During Honey Bee Embryogenesis and Metamorphosis. 1992;37-43.

    Not in our library

20. 20.

    Shuel RW, Dixon SE. Respiration in developing honeybee larvae. J Apicultural Res. 1968;7:11-19.

    Not in our library

21. 21.

    Stabe HA. The rate of growth of worker, drone and queen larvae of the honeybee, Apis mellifera. J Economic Entomol. 1930;23:447-453.

    Not in our library

22. 22.

    Tu M-P, Yin C-M, Tatar M. Mutations in insulin signaling pathway alter juvenile hormone synthesis in Drosophila melanogaster. General Comparative Endocrinol. 2005;142:347-356.

    Not in our library10.1016/j.ygcen.2005.02.009

23. 23.

    Wang D-I. Growth rates of young queen and worker honeybee larvae. J Apicultural Res. 1965;4:3-5.

    Not in our library

24. 24.

    Weaver N. Effects of larval honey bee age on dimorphic differentiation of the female honeybee. Ann Entomol Soc Am. 1957;50:283-294.

    Not in our library

25. 25.

    Wheeler DE. The role of nourishment in oogenesis. Annu Rev Entomol. 1996;41:407-431.

    Not in our library10.1146/annurev.en.41.010196.002203

26. 26.

    Wilson EO. The Insect Societies. 1971.

    Not in our library

27. 27.

    Wirtz P. Differentiation in the honey bee larva. Meded. Landbouwhogesch. Wageningen. 1973;73–75:1-66.

    Not in our library

Cited by papers we hold 5

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• Eat to reproduce: a key role for the insulin signaling pathway in adult insects · 2013
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• More than two decades of research on insect neuropeptide GPCRs: an overview · 2012
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• Molecular determinants of caste differentiation in the highly eusocial honeybee Apis mellifera · 2007
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• The Making of a Queen: TOR Pathway Is a Key Player in Diphenic Caste Development · 2007
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