Distinct conformational states of SARS-CoV-2 spike protein

Abstract

A dynamic viral spike Efforts to protect human cells against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have focused on the trimeric spike (S) protein. Several structures have shown a stabilized ectodomain of the spike in its prefusion conformation. Cai et al. now provide insight into the structural changes in the S protein that result in the fusion of the viral and host cell membranes. They purified full-length S protein and determined cryo–electron microscopy structures of both the prefusion and postfusion conformations. These structures add to our understanding of S protein function and could inform vaccine design. Science , this issue p. 1586

References 57

1. 1.

   de Wit E., van Doremalen N., Falzarano D., Munster V. J., SARS and MERS: Recent insights into emerging coronaviruses. Nat. Rev. Microbiol. 14, 523–534 (2016 ).

   Not in our library10.1038/nrmicro.2016.81

2. 2.

   Zhong N. S., Zheng B. J., Li Y. M., Poon L. L. M., Xie Z. H., Chan K. H., Li P. H., Tan S. Y., Chang Q., Xie J. P., Liu X. Q., Xu J., Li D. X., Yuen K. Y., Peiris J. S. M., Guan Y., Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong, People’s Republic of China, in February, 2003. Lancet 362, 1353–1358 (2003 ).

   Not in our library10.1016/s0140-6736(03)14630-2

3. 3.

   Hijawi B., Abdallat M., Sayaydeh A., Alqasrawi S., Haddadin A., Jaarour N., Alsheikh S., Alsanouri T., Novel coronavirus infections in Jordan, April 2012: Epidemiological findings from a retrospective investigation. East. Mediterr. Health J. 19, S12–S18 (2013 ).

   Not in our library10.26719/2013.19.supp1.s12

4. 4.

   Corman V. M., Muth D., Niemeyer D., Drosten C., Hosts and sources of endemic human coronaviruses. Adv. Virus Res. 100, 163–188 (2018 ).

   Not in our library10.1016/bs.aivir.2018.01.001

5. 5.

   Guan Y., Zheng B. J., He Y. Q., Liu X. L., Zhuang Z. X., Cheung C. L., Luo S. W., Li P. H., Zhang L. J., Guan Y. J., Butt K. M., Wong K. L., Chan K. W., Lim W., Shortridge K. F., Yuen K. Y., Peiris J. S., Poon L. L., Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China. Science 302, 276–278 (2003 ).

   Not in our library10.1126/science.1087139

6. 6.
   Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus · 2017

   No notice
7. 7.
   Middle East Respiratory Syndrome Coronavirus (MERS-CoV) origin and animal reservoir · 2016

   No notice
8. 8.
   Bats and Coronaviruses · 2019

   No notice
9. 9.
   A pneumonia outbreak associated with a new coronavirus of probable bat origin · 2020

   No notice
10. 10.
    Mechanisms of Coronavirus Cell Entry Mediated by the Viral Spike Protein · 2012

    No notice
11. 11.

    Rand R. P., Parsegian V. A., Physical force considerations in model and biological membranes. Can. J. Biochem. Cell Biol. 62, 752–759 (1984 ).

    Not in our library10.1139/o84-097

12. 12.

    Parsegian V. A., Fuller N., Rand R. P., Measured work of deformation and repulsion of lecithin bilayers. Proc. Natl. Acad. Sci. U.S.A. 76, 2750–2754 (1979 ).

    Not in our library10.1073/pnas.76.6.2750

13. 13.

    Harrison S. C., Viral membrane fusion. Virology 479-480, 498–507 (2015 ).

    Not in our library10.1016/j.virol.2015.03.043

14. 14.

    Kielian M., Mechanisms of virus membrane fusion proteins. Annu. Rev. Virol. 1, 171–189 (2014 ).

    Not in our library10.1146/annurev-virology-031413-085521

15. 15.

    Weissenhorn W., Dessen A., Calder L. J., Harrison S. C., Skehel J. J., Wiley D. C., Structural basis for membrane fusion by enveloped viruses. Mol. Membr. Biol. 16, 3–9 (1999 ).

    Not in our library10.1080/096876899294706

16. 16.

    Du L., He Y., Zhou Y., Liu S., Zheng B.-J., Jiang S., The spike protein of SARS-CoV—A target for vaccine and therapeutic development. Nat. Rev. Microbiol. 7, 226–236 (2009 ).

    Not in our library10.1038/nrmicro2090

17. 17.

    Bosch B. J., van der Zee R., de Haan C. A., Rottier P. J., The coronavirus spike protein is a class I virus fusion protein: Structural and functional characterization of the fusion core complex. J. Virol. 77, 8801–8811 (2003 ).

    Not in our library10.1128/jvi.77.16.8801-8811.2003

18. 18.

    Hoffmann M., Kleine-Weber H., Schroeder S., Krüger N., Herrler T., Erichsen S., Schiergens T. S., Herrler G., Wu N.-H., Nitsche A., Müller M. A., Drosten C., Pöhlmann S., SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. Cell 181, 271–280.e8 (2020 ).

    Not in our library10.1016/j.cell.2020.02.052

19. 19.

    Millet J. K., Whittaker G. R., Host cell entry of Middle East respiratory syndrome coronavirus after two-step, furin-mediated activation of the spike protein. Proc. Natl. Acad. Sci. U.S.A. 111, 15214–15219 (2014 ).

    Not in our library10.1073/pnas.1407087111

20. 20.

    Tortorici M. A., Veesler D., Structural insights into coronavirus entry. Adv. Virus Res. 105, 93–116 (2019 ).

    Not in our library10.1016/bs.aivir.2019.08.002

21. 21.
    A new coronavirus associated with human respiratory disease in China · 2020

    Corrected
22. 22.

    Wrapp D., Wang N., Corbett K. S., Goldsmith J. A., Hsieh C.-L., Abiona O., Graham B. S., McLellan J. S., Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science 367, 1260–1263 (2020 ).

    Not in our library10.1126/science.abb2507

23. 23.

    Walls A. C., Park Y.-J., Tortorici M. A., Wall A., McGuire A. T., Veesler D., Structure, function, and antigenicity of the SARS-CoV-2 spike glycoprotein. Cell 181, 281–292.e6 (2020 ).

    Not in our library10.1016/j.cell.2020.02.058

24. 24.

    R. Henderson, R. Henderson, R. J. Edwards, K. Mansouri, K. Janowska, V. Stalls, S. Gobeil, M. Kopp, A. Hsu, M. Borgnia, R. Parks, B. F. Haynes, P. Acharya, Controlling the SARS-CoV-2 spike glycoprotein conformation. bioRxiv 2020.05.18.102087 [Preprint]. 18 May 2020;.

    Not in our library10.1101/2020.05.18.102087

25. 25.

    Lan J., Ge J., Yu J., Shan S., Zhou H., Fan S., Zhang Q., Shi X., Wang Q., Zhang L., Wang X., Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor. Nature 581, 215–220 (2020 ).

    Not in our library10.1038/s41586-020-2180-5

26. 26.
    Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2 · 2020

    No notice
27. 27.

    Shang J., Ye G., Shi K., Wan Y., Luo C., Aihara H., Geng Q., Auerbach A., Li F., Structural basis of receptor recognition by SARS-CoV-2. Nature 581, 221–224 (2020 ).

    Not in our library10.1038/s41586-020-2179-y

28. 28.

    Wang Q., Zhang Y., Wu L., Niu S., Song C., Zhang Z., Lu G., Qiao C., Hu Y., Yuen K.-Y., Wang Q., Zhou H., Yan J., Qi J., Structural and functional basis of SARS-CoV-2 entry by using human ACE2. Cell 181, 894–904.e9 (2020 ).

    Not in our library10.1016/j.cell.2020.03.045

29. 29.

    Walls A. C., Tortorici M. A., Snijder J., Xiong X., Bosch B.-J., Rey F. A., Veesler D., Tectonic conformational changes of a coronavirus spike glycoprotein promote membrane fusion. Proc. Natl. Acad. Sci. U.S.A. 114, 11157–11162 (2017 ).

    Not in our library10.1073/pnas.1708727114

30. 30.
    Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2 · 2018

    No notice
31. 31.

    Schroth-Diez B., Ludwig K., Baljinnyam B., Kozerski C., Huang Q., Herrmann A., The role of the transmembrane and of the intraviral domain of glycoproteins in membrane fusion of enveloped viruses. Biosci. Rep. 20, 571–595 (2000 ).

    Not in our library10.1023/a:1010415122234

32. 32.

    Bosch B. J., de Haan C. A., Smits S. L., Rottier P. J., Spike protein assembly into the coronavirion: Exploring the limits of its sequence requirements. Virology 334, 306–318 (2005 ).

    Not in our library10.1016/j.virol.2005.02.001

33. 33.

    Lontok E., Corse E., Machamer C. E., Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site. J. Virol. 78, 5913–5922 (2004 ).

    Not in our library10.1128/jvi.78.11.5913-5922.2004

34. 34.

    Petit C. M., Melancon J. M., Chouljenko V. N., Colgrove R., Farzan M., Knipe D. M., Kousoulas K. G., Genetic analysis of the SARS-coronavirus spike glycoprotein functional domains involved in cell-surface expression and cell-to-cell fusion. Virology 341, 215–230 (2005 ).

    Not in our library10.1016/j.virol.2005.06.046

35. 35.

    Ye R., Montalto-Morrison C., Masters P. S., Genetic analysis of determinants for spike glycoprotein assembly into murine coronavirus virions: Distinct roles for charge-rich and cysteine-rich regions of the endodomain. J. Virol. 78, 9904–9917 (2004 ).

    Not in our library10.1128/jvi.78.18.9904-9917.2004

36. 36.

    Hurtley S. M., Bole D. G., Hoover-Litty H., Helenius A., Copeland C. S., Interactions of misfolded influenza virus hemagglutinin with binding protein (BiP). J. Cell Biol. 108, 2117–2126 (1989 ).

    Not in our library10.1083/jcb.108.6.2117

37. 37.
    RELION: Implementation of a Bayesian approach to cryo-EM structure determination · 2012

    No notice
38. 38.
    Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis · 2018

    Corrected
39. 39.
    SARS-CoV-2 spike-protein D614G mutation increases virion spike density and infectivity · 2020

    No notice
40. 40.
    The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types · 2021

    No notice
41. 41.

    Danieli T., Pelletier S. L., Henis Y. I., White J. M., Membrane fusion mediated by the influenza virus hemagglutinin requires the concerted action of at least three hemagglutinin trimers. J. Cell Biol. 133, 559–569 (1996 ).

    Not in our library10.1083/jcb.133.3.559

42. 42.

    C. Liu, Y. Yang, Y. Gao, C. Shen, B. Ju, C. Liu, X. Tang, J. Wei, X. Ma, W. Liu, S. Xu, Y. Liu, J. Yuan, J. Wu, Z. Liu, Z. Zhang, P. Wang, L. Liu, Viral architecture of SARS-CoV-2 with post-fusion spike revealed by cryo-EM. bioRxiv 2020.03.02.972927 [Preprint]. 5 March 2020;.

    Not in our library10.1101/2020.03.02.972927

43. 43.
    Development of an inactivated vaccine candidate for SARS-CoV-2 · 2020

    No notice
44. 44.

    F. Wu, A. Wang, M. Liu, Q. Wang, J. Chen, S. Xia, Y. Ling, Y. Zhang, J. Xun, L. Lu, S. Jiang, H. Lu, Y. Wen, J. Huang, Neutralizing antibody responses to SARS-CoV-2 in a COVID-19 recovered patient cohort and their implications. medRxiv 2020.03.30.20047365 [Preprint]. 20 April 2020;.

    Not in our library10.1101/2020.03.30.20047365

45. 45.

    McLellan J. S., Chen M., Joyce M. G., Sastry M., Stewart-Jones G. B. E., Yang Y., Zhang B., Chen L., Srivatsan S., Zheng A., Zhou T., Graepel K. W., Kumar A., Moin S., Boyington J. C., Chuang G.-Y., Soto C., Baxa U., Bakker A. Q., Spits H., Beaumont T., Zheng Z., Xia N., Ko S.-Y., Todd J.-P., Rao S., Graham B. S., Kwong P. D., Structure-based design of a fusion glycoprotein vaccine for respiratory syncytial virus. Science 342, 592–598 (2013 ).

    Not in our library10.1126/science.1243283

46. 46.

    Frey G., Chen J., Rits-Volloch S., Freeman M. M., Zolla-Pazner S., Chen B., Distinct conformational states of HIV-1 gp41 are recognized by neutralizing and non-neutralizing antibodies. Nat. Struct. Mol. Biol. 17, 1486–1491 (2010 ).

    Not in our library10.1038/nsmb.1950

47. 47.

    Skehel J. J., Wiley D. C., Receptor binding and membrane fusion in virus entry: The influenza hemagglutinin. Annu. Rev. Biochem. 69, 531–569 (2000 ).

    Not in our library10.1146/annurev.biochem.69.1.531

48. 48.

    Frey G., Peng H., Rits-Volloch S., Morelli M., Cheng Y., Chen B., A fusion-intermediate state of HIV-1 gp41 targeted by broadly neutralizing antibodies. Proc. Natl. Acad. Sci. U.S.A. 105, 3739–3744 (2008 ).

    Not in our library10.1073/pnas.0800255105

49. 49.

    Kovacs J. M., Nkolola J. P., Peng H., Cheung A., Perry J., Miller C. A., Seaman M. S., Barouch D. H., Chen B., HIV-1 envelope trimer elicits more potent neutralizing antibody responses than monomeric gp120. Proc. Natl. Acad. Sci. U.S.A. 109, 12111–12116 (2012 ).

    Not in our library10.1073/pnas.1204533109

50. 50.

    Chen J., Kovacs J. M., Peng H., Rits-Volloch S., Lu J., Park D., Zablowsky E., Seaman M. S., Chen B., Effect of the cytoplasmic domain on antigenic characteristics of HIV-1 envelope glycoprotein. Science 349, 191–195 (2015 ).

    Not in our library10.1126/science.aaa9804

51. 51.

    Zheng S. Q., Palovcak E., Armache J.-P., Verba K. A., Cheng Y., Agard D. A., MotionCor2: Anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Nat. Methods 14, 331–332 (2017 ).

    Not in our library10.1038/nmeth.4193

52. 52.

    Rohou A., Grigorieff N., CTFFIND4: Fast and accurate defocus estimation from electron micrographs. J. Struct. Biol. 192, 216–221 (2015 ).

    Not in our library10.1016/j.jsb.2015.08.008

53. 53.
    SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM · 2019

    No notice
54. 54.

    Pettersen E. F., Goddard T. D., Huang C. C., Couch G. S., Greenblatt D. M., Meng E. C., Ferrin T. E., UCSF Chimera—A visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605–1612 (2004 ).

    Not in our library10.1002/jcc.20084

55. 55.
    Features and development of Coot · 2010

    No notice
56. 56.
    Collaboration gets the most out of software · 2013

    No notice
57. 57.
    PHENIX : a comprehensive Python-based system for macromolecular structure solution · 2010

    No notice

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