Cross-Laboratory Analysis of Brain Cell Type Transcriptomes with Applications to Interpretation of Bulk Tissue Data

Abstract

Establishing the molecular diversity of cell types is crucial for the study of the nervous system. We compiled a cross-laboratory database of mouse brain cell type-specific transcriptomes from 36 major cell types from across the mammalian brain using rigorously curated published data from pooled cell type microarray and single-cell RNA-sequencing (RNA-seq) studies. We used these data to identify cell type-specific marker genes, discovering a substantial number of novel markers, many of which we validated using computational and experimental approaches. We further demonstrate that summarized expression of marker gene sets (MGSs) in bulk tissue data can be used to estimate the relative cell type abundance across samples. To facilitate use of this expanding resource, we provide a user-friendly web interface at www.neuroexpresso.org .

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• A single-cell atlas of the human substantia nigra reveals cell-specific pathways associated with neurological disorders · 2020
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• CSF1R inhibitor JNJ-40346527 attenuates microglial proliferation and neurodegeneration in P301S mice · 2019
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• Transcriptional profiling of microglia; current state of the art and future perspectives · 2019
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• Early life stress alters transcriptomic patterning across reward circuitry in male and female mice · 2019
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• Neuropathological and transcriptomic characteristics of the aged brain · 2017
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