Natural CMT2 Variation Is Associated With Genome-Wide Methylation Changes and Temperature Seasonality

Abstract

As Arabidopsis thaliana has colonized a wide range of habitats across the world it is an attractive model for studying the genetic mechanisms underlying environmental adaptation. Here, we used public data from two collections of A. thaliana accessions to associate genetic variability at individual loci with differences in climates at the sampling sites. We use a novel method to screen the genome for plastic alleles that tolerate a broader climate range than the major allele. This approach reduces confounding with population structure and increases power compared to standard genome-wide association methods. Sixteen novel loci were found, including an association between Chromomethylase 2 (CMT2) and temperature seasonality where the genome-wide CHH methylation was different for the group of accessions carrying the plastic allele. Cmt2 mutants were shown to be more tolerant to heat-stress, suggesting genetic regulation of epigenetic modifications as a likely mechanism underlying natural adaptation to variable temperatures, potentially through differential allelic plasticity to temperature-stress.

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Cited by papers we hold 4

• Plant Responses to Heat Stress: Physiology, Transcription, Noncoding RNAs, and Epigenetics · 2020
  No notice
• The evolution of CHROMOMETHYLASES and gene body DNA methylation in plants · 2017
  No notice
• Genetic and epigenetic control of plant heat responses · 2015
  No notice
• DNA methylation in Arabidopsis has a genetic basis and shows evidence of local adaptation · 2015
  No notice

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