Regulation of denitrification at the cellular level: a clue to the understanding of N 2 O emissions from soils

1Human activities have more than doubled the annual input of reactive nitrogen to the biosphere compared with prehistoric rates. This anthropogenic reactive N is primarily fertilizer N, biologically fixed N (in legume crops) and NOx from combustion. The ongoing amplification of the global N cycle has already altered the biogeochemical processes of natural ecosystems, their trophic dynamics and biological diversity. The ultimate fate of anthropogenic nitrogen is a return to the atmosphere, either as N2, N2O or NO, which are the gaseous products of microbial red/ox-transformations of mineral nitrogen. The N-gas product-stoichiometry of these processes is controlled by the ecology and regulatory biology of the organisms involved, as modulated by environmental factors. A better understanding and quantification of these processes is urgently needed to improve our chances to minimize N2O emissions. The current accumulation of N2O in the atmosphere accounts for approximately 9 per cent of estimated anthropogenic global warming and is recognized as the major factor causing destruction of stratospheric ozone.

2The global N2O emissions from cultivated soils have been estimated at 3.3 Tg N2O yr−1, which is around 50 per cent of the estimated global anthropogenic N2O emission. A substantial part of the remaining anthropogenic N2O emissions (from water bodies and uncultivated areas) are driven by nitrogen losses from agroecosystems as well. There is thus little doubt that terrestrial habitats (both cultivated and uncultivated) are one of the most important sources of atmospheric N2O. Denitrification is probably a much more potent N2O source than nitrification, as indicated by the low N2O/NO3− product stoichiometry of nitrification. Tracer studies suggesting a major role of nitrification may possibly have confounded coupled nitrification–denitrification via nitrite.

3Few tools are available to reduce the emissions from natural ecosystems, apart from reducing the inputs of anthropogenic reactive nitrogen. The effect of such reductions may be slow, however, owing to the long residence time of reactive nitrogen (as biomass and soil organic N) within the systems. Cultivated areas, on the other hand, are intensively managed by drainage, cropping, tillage, liming, fertilization and use of agrochemicals. These operations should hold a potential for reducing the N2O emissions, provided that we know how they affect the activity of the organisms responsible for N2O production in the soil. Despite several decades of research on N2O emissions, however, few (if any) practical ways to achieve substantial reductions of N2O emissions have been invented.

4Why is there so little progress? How can we improve our efforts? We suspect that one of the reasons for the current shortcoming of the research on N2O emission is that there has been too little cross-talk between disciplines. Great progress has been achieved from studies of the biochemistry, regulatory biology and physiology of denitrifying prokaryotes over the last two decades, driven by molecular biology. Yet, too little of this knowledge has reached the agronomists/biogeochemists/earth scientists who have made great progress within their own fields. Most experiments within each of the disciplines mentioned are designed to solve ‘disciplinary’ problems, by scientists with only rudimentary understanding of how adjacent disciplines think and work, and their recent progress. We believe that better interaction and discussions between the disciplines hold a potential for progress in the search for ways to mitigate N2O emission.

5The present paper is an attempt to illustrate this potential, by reviewing recent progress in a series of experimental approaches dedicated to understanding the mechanisms at the cellular level which control the N2O/(N2+N2O) product ratio of denitrification. This ratio is of utmost importance for the earth sciences in their attempt to link N2O emissions to the current amplification of the nitrogen cycle. Before diving into the microbiological investigations, we need to briefly recapitulate some research on N2O emissions within the earth sciences.

6N2O emissions from agricultural soils have been studied intensively in numerous field trials on all continents, reviewed and analysed thoroughly by Stehfest & Bouwman. Excess fertilizer N doses result in high emissions, but N2O emissions correlate only poorly with the fertilizer N level when within realistic ranges. Numerous other factors are involved in controlling the N2O emission from soils, which contribute to the huge spatio-temporal variation in emissions in field trials. An unfortunate consequence of the spatio-temporal variation is that the power of statistical tests of such experiments is low, i.e. although the experimental treatments may have a real effect, there is a high risk of erroneously concluding that there was no effect (type II error). A number of management effects may have been erroneously rejected for this reason. A simple calculation of the confidence interval of the difference between contrasting treatments could reveal the pitfall; i.e. the substantial effect of a management could have existed without being detected as statistically significant. This is often neglected, possibly because it is frustrating to illuminate the low statistical power of the experiment.

7Another problem with field trials is that the observations tend to be ‘anecdotal’ because the effects of specific operations on N2O emissions are likely to be conditional, i.e. dependent on several other variables such as soil type, climate and other management operations. Drainage of the soils is a good example: drainage of paddy rice soils (drainage is often included in the annual management of such fields) will enhance the N2O emission; in farmed organic soils, the lowering of the water table (i.e. drainage) may or may not reduce N2O emissions depending on other factors, whereas adequate artificial drainage to sustain a low water table depth throughout the whole year appears to reduce N2O emissions from grassland soils. Another example is tillage, which may result in higher or lower annual N2O emissions, apparently depending on the climate and possibly a number of soil variables ( and references therein).

8A third shortcoming of nearly all field experiments is that the N2O/N2 product ratio has not been measured, primarily because it has proved difficult to obtain reliable field measurements of N2 production. The acetylene inhibition method may be used, but is likely to underestimate denitrification owing to diffusion limitation and acetylene-catalyzed oxidation of NO. Tracer-based measurements are possible, but have not been used much because they are complicated, demanding continuous monitoring of both gas emissions and the tracer (15N) concentration in the nitrate/nitrite pool of the soil. The scarcity of reliable measurements of N2 production in field experiments is most unfortunate, since the N2O/N2 product ratios should be a target for designing mitigation options.

9The potential benefit of measuring both N2 and N2O production is illustrated by two studies of intact soils from field experiments conducted by Zaman et al., where they quantified both N2O and N2 production (the latter by the acetylene inhibition technique). They found that liming invariably reduced the N2O/N2 product ratio, and the effect on net N2O emission was either nil or positive (higher emission from limed soil). Their quantification of N2 production by the acetylene inhibition method may have been biased for the reason mentioned earlier (lack of steady-state conditions owing to restricted diffusion, and NO scavenging by acetylene). Nevertheless, their observation of an altered N2O/N2 product ratio is probably valid since we may assume that the bias is the same in all treatments.

10Biogeochemical modelling of denitrification and its N2O production are based on relatively crude models of the process and its regulation at the cellular level. Much effort has been invested in modelling the heat-, water- and not the least oxygen-transport within the soil matrix. Less effort has been invested in refining the modelling of denitrifying community phenotype, which is a ‘black box’ in most models, based on relatively antique parameters for enzyme and growth kinetics. In addition, all soils are assumed to harbour the same community phenotype. It appears bluntly wrong to assume that all community phenotypes are equal, as judged from investigations of community phenotypes of contrasting soils. Despite these (and other) gross simplifications, the simulated annual N2O emissions are often in reasonable agreement with measured values for contrasting soils and for contrasting soil management scenarios, although the temporal variation is still a challenge.

11Simplifications are necessary in biogeochemical ecosystem modelling, but it seems likely that the existing models could be improved through a dialogue between the modellers and the research community working with the genetic and physiological basis of denitrification. This is particularly true if the purpose of the modelling is to design ‘mitigation measures’, i.e. soil and fertilization routines that reduce the N2O emissions. As stated previously, a reduction in the N2O/(N2 + N2O) product ratio of denitrification should be a target when searching for mitigation measures. The existing models are possibly inadequate for this purpose, although this is unknown because we lack reliable data for N2 production in field experiments to test their predictions. It would be arrogant (but possibly legitimate) to claim that when a nearly perfect match is found between measured and simulated annual N2O emissions, the biogeochemical model is right for a number of wrong reasons. It is less arrogant (but fully legitimate) to claim that if used to simulate phenomena for which the models are not rigorously tested, their predictions are rather hypothetical.

12Our impression is that field experiments and modelling of N2O emissions have not made much progress regarding mitigation of N2O emissions. This is reflected in several reviews of available mitigation options, which generally recommend operations that (i) secure synchronization of mineralization with the need for mineral N by growing crops and (ii) minimize the mineral N concentrations in the soil during off-season, which are no more than the general recommendations for good agronomic practice to minimize nitrate leaching. A perpetuation of field experiments and modelling of N2O emissions along the same track will probably not result in substantial progress regarding mitigations. That is not to say that such activities should be terminated. Field emission data are extremely important for several obvious reasons, and any hypothetical mitigation must be tested in field experiments. We are convinced that if there exist novel ways to reduce N2O emissions, such mitigation options are more likely to be found through studies of the ecology and regulatory biology of denitrifying prokaryotes, with a deliberate focus on their ability to express N2O reductase. We have dedicated ourselves to work along these lines, and the rest of this paper is a first attempt to summarize some of our findings.

13Denitrification is the stepwise reduction of NO3− through NO2−, NO, N2O to N2, driven by four reductase enzymes NAR/NAP, NIR, NOR and N2OR, respectively. This enables the organisms to sustain respiratory metabolism during oxygen limitation, with NOx as terminal electron acceptors. The denitrification proteome (NAR, NIR, NOR and N2OR plus several other proteins) is synthesized in response to oxygen depletion, and is blocked by high oxygen concentrations (both transcriptional and post-transcriptional control).

14The transcription of the genes coding for the individual reductases is controlled by a network of transcriptional regulators and ancillary factors which respond to intra- and extracellular signals among which are oxygen and N-oxides (NO and NO2−). Although important ‘nuts and bolts’ of this regulatory network have been unravelled for a few model strains, such as Paracoccus denitrificans, much remains to be discovered before we are able to fully understand the phenotypic response. For instance, we have found that phenotypic responses of various mutants of P. denitrificans suggest that the transcription of nar requires both FnrP and NarR (dual control), while the transcription of nosZ (coding for N2OR) is equally effective with only FnrP (in response to oxygen depletion) or NNR (responding to NO). Another conspicuous finding regarding the regulation in P. denitrificans is that N2OR is expressed much earlier than NIR and NOR (and possibly NAR), as illustrated in figure 1. Moreover, only a fraction of the cells are actually able to express NIR and NOR in due time before all the oxygen has been depleted. We hypothesize that this is due to a stochastic initiation of the transcription of nir and nor, which then accelerates owing to product stimulation (NO accelerates transcription of both genes). In contrast, nearly 100 per cent of the cells appear to express N2OR, as judged from the specific rate of reduction of externally supplied N2O.

15The peculiar patterns of regulation in P. denitrificans explains why this organism emits only traces of N2O (less than 2 nmol flask−1) during transition from oxic to anoxic conditions (figure 2).

16The denitrification phenotype of P. denitrificans at pH 7 demonstrates an outstanding performance regarding efficient reduction of NOx all the way to N2, with only minor emissions of both NO and N2O (less than 2 nmol N2O flask, or less than 0.004% of all NO3-N reduced to N2). If the denitrifying communities of soils performed equally well, their contribution to emission of NO and N2O would be negligible. The performance of P. denitrificans appears to be exceptional, however. A strain that has been studied in equal detail in our laboratory is the soil bacterium Agrobacterium tumefaciens. This organism is unable to reduce N2O to N2 because it lacks nosZ, i.e. the gene coding for N2OR. Strains which lack nosZ occur within many genera of denitrifying prokaryotes, and if organisms with such truncated denitrification apparatus were dominating in soils, it would obviously result in high N2O/(N2+N2O) product ratios of denitrification. But A. tumefaciens contrasts with P. denitrificans in another conspicuous way: when confronted with a rapid transition from oxic to anoxic conditions, it appears unable to perform a balanced expression of NIR and NOR, resulting in extremely high emissions of NO, as illustrated in figure 3.

17We have also analysed the denitrification regulatory phenotype (DRP) of a number of reference strains and recently isolated strains within the genus Thauera under nearly identical experimental conditions as that for P. denitrificans shown in figure 2. One of the strains lacked nosZ and produced 100 per cent N2O, while the seven other strains had all the necessary genes to make a complete denitrification from NO3− to N2. A common feature of all the Thauera strains was robust control of NO at nanomolar levels, very similar to P. denitrificans (5–35 nM in the liquid phase). They deviated grossly, however, regarding the transient accumulation of both NO2− and N2O. Some of the strains reduced all NO3− to NO2− before expressing NIR and NOR, wheras others accumulated negligible amounts of NO2−. The strains were also different regarding the transient accumulation of N2O, ranging from 0.06 to 5 per cent of all NO3−-N finally reduced to N2. The results are summarized in figure 4, together with their phylogenetic relationship based on 16S rDNA.

18There exists a plethora of similar studies where the transient accumulation of N2O has been studied in a similar way for complex communities, biofilms and in cultured denitrifiers. It is difficult to summarize these results in a consistent way, since the experimental conditions are widely different from one experiment to the other. But the general impression is that the denitrification phenotypes of prokaryotes are profoundly different regarding their ‘intrinsic’ propensity of emitting N2O, as assessed by the amounts of N2O accumulating transiently when switching from oxic to anoxic respiration. These differences between denitrification phenotypes are most probably owing to differences in the regulatory network controlling the transcription of their denitrification genes (and possibly post-translational regulation). Thus, we propose a term ‘DRP’, which is a set of variables that characterize the organisms ability to perform a balanced and effective transition from oxic to anoxic respiration with marginal emissions of intermediates.

19Assuming that denitrifying bacteria are profoundly different regarding their DRPs, a crucial question is whether these characteristics are in any way related to phylogeny of the organisms. If such relationships exist, the analysis of denitrification community DNA would possibly enable us to predict characteristics of the community phenotype, although the task would be far from trivial owing to interactions via the intermediates NO2− and NO. Further on, the phylogeny of the DRP could also shed some light on the evolution of the various DRP traits and their fitness value.

20However, we have scant knowledge about the phylogeny of various DRP traits, since this has hardly been studied at all in the past. The results already mentioned for the closely related strains within Thauera demonstrated surprisingly different DRPs among such closely related strains, both regarding their transient accumulation of NO2− and N2O, but not for NO. The allocation of these DRPs within the phylogenetic tree does not suggest any systematic relationship between phylogeny and the various DRP traits.

21To sum up, it seems clear that denitrification phenotypes are profoundly different regarding their propensity to emit N2O, assessed by their transient accumulation of N2O when initiating denitrification in response to oxygen depletion. They are also very different regarding their transient accumulation of nitrite (NO2−). We have too few rigorous comparative investigations to draw conclusions on the possible relationship between these phenotypes and taxonomy/phylogeny, but the study of closely related strains of Thauera do indicate that we may expect to find as much variation within taxonomic groups as between them. This would mean that characterizing the composition of denitrifying communities by metagenomic sequencing of genes coding for 16S rRNA or functional denitrification genes has limited value as a predictor of the communities' propensity to emit N2O. It would not imply, however, that no relationship will be found between the community composition and its propensity to emit N2O. Attempts have been made, with variable success, in ecophysiological studies of N2O formation. Although such approaches cannot be expected to reveal causal relationships between genetic composition and phenotype of soil microbial communities, they may serve as hypothesis-generating observations. In order to find causal relationships, we may have to dive deeper than to phylogeny, possibly by identifying regulatory gene sequences that characterize various DRPs across phyla.

22A recurring observation in ecological studies of denitrification has been that the product ratio of denitrification is strongly affected by pH. Soils have inherently different pH depending on their parent mineral material modulated by biological processes, and the N2O/(N2+N2O) product ratio appears to be negatively correlated with pH within the normal range for agricultural soils (pH 5–8). This would indicate that liming slightly acidic soils could be a way to reduce N2O emissions, but this has been effectively ignored until recently. Why did it take so long time to discover this? Possibly for two reasons. One is that the immediate response to liming is a drastic increase in N2O emission, most probably because it causes a transient enhancement of net N mineralization and nitrification. A second reason may be that the mechanism causing the observed pH effect on the N2O/N2 product ratio of denitrification was not well understood.

23How can the pH affect the N2O/N2 product ratio of denitrification? Our studies of the model strain P. denitrificans shed some light on this: — At pH 7, P. denitrificans emits nearly no N2O when switching from oxic to anoxic denitrification in batch cultures.— Lowering the pH of the medium resulted in increasing transient accumulation of N2O, and at pH 6, it produces nearly 100 per cent N2O (no N2O reductase activity).— The lack of N2O reductase activity at pH 6 was not caused by low relative transcription rate of nosZ compared with that of the other denitrification genes; the ratio between mRNA copy numbers for nosZ and that for nirS was practically unaffected by pH (figure 1).— Neither is it due to a particularly narrow pH range for the activity of the N2O reductase enzyme (compared with that of the other denitrification enzymes): N2O reductase expressed at pH 7 was functioning well at pH 6 when tested in vivo.Our tentative conclusion is that low pH hinders the synthesis of a functional N2O reductase enzyme, most probably by interfering with the assembly of the enzyme in the periplasm (which is the location of the functional enzyme).

24— At pH 7, P. denitrificans emits nearly no N2O when switching from oxic to anoxic denitrification in batch cultures.

25— Lowering the pH of the medium resulted in increasing transient accumulation of N2O, and at pH 6, it produces nearly 100 per cent N2O (no N2O reductase activity).

26— The lack of N2O reductase activity at pH 6 was not caused by low relative transcription rate of nosZ compared with that of the other denitrification genes; the ratio between mRNA copy numbers for nosZ and that for nirS was practically unaffected by pH (figure 1).

27— Neither is it due to a particularly narrow pH range for the activity of the N2O reductase enzyme (compared with that of the other denitrification enzymes): N2O reductase expressed at pH 7 was functioning well at pH 6 when tested in vivo.

28The knee-jerk reaction of many microbial ecologists to this is that P. denitrificans is no more than a model strain with little relevance to natural habitats. However, we have collected convincing evidence that the phenomenon occurs in soils: — Soils from long-term liming experiments demonstrate a pervasive control of pH on the (N2O/N2 + N2O) product ratio, although the relative amount of nosZ genes and their transcripts (compared with that of the other denitrification genes) appears unaffected by pH within the range 5–8.— Experiments with cells extracted from the same soils (long-term liming experiments) show essentially the same phenomenon for cells from neutral soils, but cells from acid soils appear unable to produce functional N2O reductase even when transferred to neutral conditions.— Experiments with cells extracted from organic soils with different cultivation history, pH and N2O emissions, demonstrated the same pH dependency, although the contrasting community composition appeared to modulate the pH sensitivity.— All strains (Gram positives and Gram negatives) tested in our laboratory so far have shown the same pH dependency of the relative activity.— Ongoing investigations of soils sampled from long-term field experiments (Austria, Hungary, Nepal and China) demonstrate invariably that the (N2O/N2 + N2O) product ratio of denitrification under anoxic incubation of intact soils is negatively correlated with pH, independent of the mechanism controlling the soil pH (liming, parent material or excess fertilization).

29— Soils from long-term liming experiments demonstrate a pervasive control of pH on the (N2O/N2 + N2O) product ratio, although the relative amount of nosZ genes and their transcripts (compared with that of the other denitrification genes) appears unaffected by pH within the range 5–8.

30— Experiments with cells extracted from the same soils (long-term liming experiments) show essentially the same phenomenon for cells from neutral soils, but cells from acid soils appear unable to produce functional N2O reductase even when transferred to neutral conditions.

31— Experiments with cells extracted from organic soils with different cultivation history, pH and N2O emissions, demonstrated the same pH dependency, although the contrasting community composition appeared to modulate the pH sensitivity.

32— All strains (Gram positives and Gram negatives) tested in our laboratory so far have shown the same pH dependency of the relative activity.

33— Ongoing investigations of soils sampled from long-term field experiments (Austria, Hungary, Nepal and China) demonstrate invariably that the (N2O/N2 + N2O) product ratio of denitrification under anoxic incubation of intact soils is negatively correlated with pH, independent of the mechanism controlling the soil pH (liming, parent material or excess fertilization).

34Our investigations demonstrate that the N2O/(N2+N2O) product ratio of denitrification is pervasively controlled by pH, be it in pure cultures of denitrifying bacteria or in soils. Our in-depth studies of the model strain P. denitrificans showed that the effect is primarily owing to interference with the assembly of the enzyme, rather than a narrow pH range for the N2O reductase enzyme. The recurring observations of similar pH effect on denitrification in soils indicate that it is a general phenomenon in nature. The findings suggest that the ongoing soil acidification of agricultural soils by intensification of agriculture and excessive use of N-fertilizers, as convincingly demonstrated for China, may enhance the N2O emissions drastically. We hypothesize that careful adjustment of pH in agricultural soils will reduce N2O emissions from slightly acidic soils. This needs to be tested rigorously in field trials, however. More sophisticated ways to reduce N2O emissions may emerge in the future as we learn more about the ecology and the regulatory biology of denitrifying prokaryotes.