Adipose-derived Stem Cell Secretome and Its Potential Role in the Treatment of Androgenetic Alopecia

INTRODUCTION: The secretory properties of white adipocytes are thought to contribute to the association between hair folliculogenesis and hair growth.1,2 We investigated the quantitative and qualitative secretome profiling of adipose derived stem cells from different zones of hair growth in patients with androgenetic alopecia. METHODS: We included six male patients, candidates for follicular unit extraction hair transplantation, all in the early stage of androgenetic alopecia. One millimeter punch samples of adipose tissue located beneath hair follicles of three scalp areas (alopecia, border-line and normal hair growth) and one periumbilical sample from each patient were enzymatically digested, centrifuged, washed, and cell pellets were ceded and maintained in culture medium until reached monolayer. Conditioned media samples were thawed and analyzed with 41plex kit. Results were registered by Luminex platform and calculated with xPonent software. RESULTS: We analyzed the levels of 35 signaling proteins. The levels of Inteleukin-6, Vascular Endothelial Growth Factor, Endothelial Growth Factor, and Eotaxin were significantly higher in the alopecia zone in comparison with the periumbilical and occipital. The similar trend was found for Monocyte Chemotactic Protein-3, Interferon gamma-inducible Protein-10, and Macrophage Inflammatory Protein-1 alpha. On the other side, Monocyte Chemoattractant Protein-1 level was the lowest in alopecia comparing with other zones. Other examined proteins did not show changes. CONCLUSIONS: The observed differences in these signaling molecules’ expression could contribute for both achieving therapeutic goals for hair loss conditions and shading more lights on the androgenetic alopecia etiology but also highlight the need to investigate adipose derived stem cells secretory proteome in all other conditions linked to hair loss. REFERENCES: 1. Schmidt B, Horsley V. Unravelling hair follicle-adipocyte communication. Exp Dermatol. 2012;21(11):827–830. doi: 10.1111/exd.12001. 2. Kruglikov IL, Zhang Z, Scherer PE. The role of immature and mature adipocytes in hair cycling. Trends Endocrinol Metab. 2019;30(2):93–105. doi: 10.1016/j.tem.2018.11.004. Epub 2018 Dec 14.

Abstract licence: CC BY-NC-ND

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