Sulfur nutrition and its role in plant growth and development

1Sulfur is one of the essential elements required by all living organisms, including plants. Sulfur is a constituent of the proteinaceous amino acids such as methionine and cysteine, glutathione, vitamins (biotin and thiamine), phytochelatins, chlorophyll, coenzyme A, and S-adenosyl-methionine. Sulfur is also involved in disulfide bond formation in proteins and enzymes’ regulation, particularly in redox control. It offers protection from oxidative damage through glutathione and its derivatives., Sulfur is also a component of several secondary metabolites (SMs) of plants and is required for the plant’s physiological functions, growth, and development. The sulfur demand in plants is dependent on the types of species and stages of development. For instance, during seed development and vegetative growth, a higher amount of sulfur is required. Sulfur-containing compounds such as Fe–S clusters-containing proteins are required in multiple biological processes, such as photosynthesis, energy generation, photoprotection, and metabolic reactions.,, The primary and dominant sulfur source is inorganic sulfate (SO42−) for the plants., Multiple transporters are involved in SO42− uptake and its transportation from source to sink. Chloroplasts of young leaves are the prominent organelle where assimilation of SO42− to cysteine occurs; however, synthesis of methionine and cysteine can also happen in seeds and roots., Moreover, in plants, sulfur, and sulfur-containing compounds are directly or indirectly take part in biotic and abiotic stress management, metabolism, and signaling. The overall role of sulfur in plant growth and development is summarized in Figure 1.

2There are various sources of sulfur found in the soil. Organic matter contains around 95% of the total sulfur content of the soil (https://blog-crop-news.extension.umn.edu/2018/01/evaluating-need-for-sulfur-in-high.html). Breakdown or decomposition of organic matter results in mineralization of organic sulfur into the SO42−, which will be available to plants. Apart from the organic matter, various minerals inside the soil also consist of a different sulfur forms. Hence, breaking down or weathering these minerals results in transforming a part of sulfur into sulfate. In the atmosphere, a higher concentration of SO2 is observed around the industrial area. Fuel-burning is also a source of sulfur. It releases sulfur in the form of sulfur dioxide (SO2). This SO2 is dissolved in rainwater and finally reaches the soil. Pesticides contribute comparatively small amounts of sulfur to the soil., However, some pesticides contain sulfur, and the use of pesticides adds sulfur to the soil. Chemical fertilizers contain a considerable amount of sulfur along with nitrogen, potassium, and phosphorus.

3Sulfur deficiency results in poor quality and yield of crops., Mild sulfur deficiency may have a negligible effect on yield but have a significant impact on quality. Thus, poor or low sulfur storage proteins are synthesized in sulfur limiting soil, such as omega-gliadin and high molecular weight subunits of glutenin at the expense of sulfur-rich proteins in wheat., It has been reported that sulfate deficiency leads to decreased synthesis of Rubisco (ribulose-1,5-biphosphate carboxylase/oxygenase) enzyme that affects the assimilation rates of CO2 which eventually results in retarded synthesis of carbohydrates this resulted in the chlorosis of young leaves. Several studies suggest that sulfur deficiency affects biomass, overall morphology, yield, and nutritional value of the plants. For instance, in Eruca sativa L sulfur deficiency leads to altered biomass production and chlorophyll synthesis. Moreover, the impacts of sulfur supplementation on grain yield and protein yield of agronomically important traits in wheat were observed in a study. The average protein yield of different cultivars was increased from 0.018 to 0.024 kg m−2 and the average grain yield of different cultivars was increased from 0.20 to 0.29 kg m−2. In another study, it has been observed that impact of sulfur supplementation improve grain yield and protein yield in agronomically important plants like wheat and oilseed rape. Additionally, sulfur deficiency leads to decreased root hydraulic conductivity as a response probably implicated with signaling nutrient starvation from root to shoot., Moreover, sulfur deficiency results in the reduction of the internal sulfur pool and an increase in the soluble nitrogen pool together with amide and nitrate as a consequence of the ratio of nitrogen and sulfur imbalance., Sulfur deficiency symptoms in economically important plants are summarized in Table 1.

4Sulfur deficiency is more prevalent in recent years because of the reduction in atmospheric inputs. Reduced industrial sulfur emissions because of pollution control regulation resulted in the reduced disposition of sulfur into the soil from the atmosphere (https://www.dekalbasgrowdeltapine.com/en-us/agronomy/the-importance-of-sulfur-for-corn-and-soybeans.html).,, In addition, extensive use of high purity and sulfur-free or low percentage sulfur-containing fertilizers/pesticides and intensive production of higher-yielding crops may also contribute to more sulfur deficiency in the soil.,,,, It is reported that between 1990 and 2011 the atmospheric concentration of SO2 has been decreased by 20 teragram. It has been reported that soil factors also affect sulfur deficiency. Organic sulfur is the primary source of sulfur utilized by plants. Therefore, the soil’s organic content is crucial, and if it is low, it will lead to a sulfur deficiency in plants. Organic sulfur becomes available to the plants through mineralization that is carried out by microorganisms., This microbial activity is dependent on the temperature of the soil as well as the moisture content. Microbial activity is reduced by cold and excessively wet or dry conditions, thereby decreasing sulfur availability from soil organic matter to the plants., The lack of sulfur can be highly variable at the field level because soil sulfur availability differs significantly from soil organic matter and texture. Sulfur deficiency is frequently seen in sandy soil, lower organic matter, and higher elevation areas of a field. However, high organic matter, lower-lying, and heavier textured areas typically have sufficient sulfur.

5There are several ways to overcome the sulfur deficiency. Chemical fertilizer, Farmyard Manure (FYM), compost, or organic matter can be used to overcome the sulfur deficiency. There are more than 20 different sulfur-containing fertilizers are available commercially which is immediately available for plant uptake. A list of percent sulfur content in different chemical fertilizers is given in Table 2.

6Ammonium thiosulfate is used with either solution of urea-ammonium nitrate or by the mixture of ammonium sulfate and urea. Sulfate of potash magnesia or potassium sulfate can be added to muriate of potash to provide sulfur and potassium. But sulfur fertilizers should be applied to crop that requires sulfur to avoid the chances of leaching from the root zone. Since these fertilizers are used before planting, sulfate can be leached from sandy soil before crop requirement. A recent study on wheat showed that the use of sulfur-containing fertilizers accelerated their germination as well as an improved immune response against pathogens.

7AMF are soil-borne fungi that colonize with plant roots. AMF form vesicles, arbuscules, and hyphae in roots, and also, they extend their hyphae in the rhizosphere. AMF works as a bio-fertilizers that improves plant growth by improving water and mineral nutrient uptake from soil rhizosphere. Several studies on AMF have emphasized their numerous advantages on crop productivity and soil health. Thus, it is believed that AMF could be considered as a substitute for inorganic chemical fertilizers.

8Fertilizers can be an option for sulfur supplements during deficiencies. However, the timing and type of sulfur application influence the presence of sulfur in the soil and the availability of the plant. AMF shows a symbiotic association with gymnosperm, angiosperm, fern, and lycopod., Intra-radical hyphae (IH) of AMF offer fungal extension inside the host plants’ cortical region. In contrast, extra-radicular hyphae (ERH) consist of three primary functions: infection of host plants, nutrient acquisition, and production of fertile spores. Many reports show that AMF colonization with plants increases the sulfur content of plants by increasing its uptake from the soil. During sulfur limitation, plants absorb SO42− very rapidly, which leads to the formation of the SO42− depletion zone. In such conditions, the AMF ERH can enlarge and extend across the region of SO42− depletion and could be a contributing factor mainly in the provision of sulfur under sulfur limitation conditions. Moreover, recent findings have shown that the colonization by AMF also influences plant sulfate transporters’ expression, thereby increasing the host plant’s sulfur content. AMF hyphae provide a large surface area compared to the plant roots, which act as an important site for microbial interactions that play an essential role in nutrient cycling. It has shown that an AMF like root endophytic fungus Serendipita indica helped maize plants to uptake sulfate, particularly under sulfur-deprived conditions.

9For soil fertility and plant viability, various microbial communities are required., It has been shown that AMF hyphae contain higher sulfonate desulfurizing bacterial communities than bulk soil. In another study, AMF inoculated with Lolium perenne showed a significant increase in the colonization of root and cultivable sulfonate mobilizing bacterial colonies, helping sulfur supply to the plant. Similarly, the addition of 2-(N-morpholine)-ethane sulfonic acid (MES) to soil has been found to stimulate not only sulfonate mobilizing bacteria but also the metabolites of this bacteria which are considered to have important role in the growth improvement of ERH of Glomus intraradices. This is essential for increasing the sulfur uptake as enriched hyphal growth arises from sulfonate mobilizing bacterial metabolites stimulates the propagation of this bacterial community in a positive feedback loop. Therefore AMF has a crucial function in plant sulfur metabolism in up-regulating plant sulfate transporters via interaction with organo-sulfur mobilizing microbes. Like the rhizosphere, the AMF hyposphere functions as a region for elevated bacterial activity and its abundance., It is not recognized whether the associated microbes transfer sulfur to the host plant and its symbiont fungi. Plant roots, mycorrhizal hyphae, and several other microbes release extracellular sulfatases into the soil rhizosphere. Although there is no direct evidence of the transfer of sulfur to the plant host through the ERH of AMF; the possibility of release of sulfur indirectly from sulfonate desulfurizing bacteria still exists and can be increased in number by staying away from its predators such as protozoa and nematodes.

10Altogether, there is a great need to understand the pathways required to mobilize sulfonates and sulfate-esters that are dominantly present as a chief source of sulfur in the soil. The humic material can be depolymerized by the saprotrophic fungi resulting in the release of sulfate-esters to fungi and bacteria, and sulfonates to the special type of bacteria consist of a monooxygenase enzyme complex. Since desulfurizing microbial populations enriches the rhizosphere and hyphosphere, and hence released SO42− gets assimilated very rapidly, resulting in a sulfur diminished region in the rhizosphere. The percentage of the root colonization and the extent of the sulfonate mobilizing bacterial community has been known to increase due to the inoculation with AMF. Therefore, crop yield can be sustainably improved by inoculation practices in those areas where sulfur is becoming a limiting factor for plant growth.

11Various membrane transporters help out plants with sulfate uptake from soil and its distribution inside plant cells. Transport across the plasma membrane is eased by a proton gradient maintained by a proton ATPase. The Symport mechanism involves the entry of H+ along with sulfate. Sulfate transportation across the tonoplast membrane is managed by the electrical gradient in-between the cytoplasm and vacuole sap. The inner membrane of the chloroplast contains an H+/ SO42−, which may mediate SO42− transport into chloroplasts.,

12It has been reported that sulfur status of plants regulates the expression of most of the sulfate transporters. According to the functional, cellular, and subcellular expression, the sulfate transporter gene family consists of five different groups.,,, Group 1: These are high-affinity sulfate transporters, and hence, these sulfate transporters are implicated with the uptake of sulfate by the roots; Group 2: These are low-affinity vascular sulfate transporters; Group 3: Transporters of this group also known as ‘leaf group’; Group 4: These transporters are associated with the sulfate uptake into the plastids before its reduction; Group 5: Role of sulfate transporters of this group is still not much studied.

13In plants, cystine is the precursor and the sulfur donor for the synthesis of various organic sulfur compounds; hence, the majority of sulfate taken up by the roots is further reduced to sulfide to convert it into cysteine., The reduction process mainly takes place in the chloroplasts, and this process involves three main steps. First, activation of sulfate to adenosine 5’-phosphosulfate (APS) in the presence of an enzyme ATP sulfurylase. Second, activated APS is reduced to sulfite using APS reductase as an enzyme and glutathione as a reductant., Third, reduction of sulfite using sulfite reductase enzyme and reduced ferredoxin as a reductant. Sulfide afterward incorporates into cysteine in a reaction catalyzed by O-acetyl serine (thiol) lyase, with O-acetyl serine as the substrate. O-acetyl serine formation is catalyzed by serine acetyltransferase and together with O-acetyl serine(thiol)lyase, associated with cysteine synthase. It has been shown that cysteine synthesis is an important reaction in the direct coupling between sulfur and nitrogen metabolism in plants., The sulfate reduction pathway is regulated by adenosine phosphosulfate reductase since this enzyme activity is lowest among the enzymes involved in the assimilatory sulfate reduction pathway., Furthermore, allosteric inhibition and metabolite activation or repressions of the genes encoding the APS reductase are involved in the expression regulation of this enzyme. Thus, both the activity and the expression of APS reductase alter quickly in response to either sulfur starvation or the presence of reduced sulfur compounds. Cysteine, glutathione, Sulfide, or O-acetyl serine are probably APS reductase regulators.,

14Though stress decreases the plant’s growth to a very low level as sulfur is limited, a detectable sulfur level in sulfur deficit plants is reported., Methionine and other major sulfur-containing amino acids use cysteine as the reduced sulfur donor for their synthesis via the so-called trans-sulfurylation pathway., Glutathione, phytochelatins, and secondary sulfur compounds also use cysteine as the direct precursor for their synthesis., The sulfide residue of cysteines in proteins plays a significant role in the binding of enzymes with the substrate, in metal-sulfur clusters in proteins (e.g., ferredoxins), and in the regulatory proteins (e.g., thioredoxins) (Figure 2).

15Most of the sulfate permeases are located in the cytoplasmic membrane. The given Transporter Classification (TC) system consists of nine classes of membrane transporters. TC system is similar to the Enzymes Classification (EC) system; hence, phylogenetic information is also incorporated into this. Most of the known sulfate permeases belong to two transporter classes that include three subclasses: eukaryotic sulfate transporters are categorized in second class, which further divides into two main subclasses (TC 2.A.47. and TC 2.A.53), while prokaryotes possess only one family that belongs to the class third (TC 3.A.1.).,

16Sulfate transporters are involved in sulfate uptake in eukaryotes, but their role in prokaryotes is not clear.,, They transport inorganic anion or perform as anion–anion exchangers. At the same time, some transporters work as sulfate-H+ or sulfate-bicarbonate symporters. Many vertebrate SulP homologs have been reported to work as anion–anion antiport. For instance, the mouse homolog, SLC26A6, can transport sulfate, oxalate, formate, bicarbonate, and chloride, exchanging any of these anions for a different one., They share different affinities with their other substrates. The transport domain of the sulfate promoter comprises 12 to 14 transmembrane helices. For the recruitment of sulfate, positive arginine residues should be present in extracellular loops., Biochemical studies revealed that sulfate permease functions can also be controlled by phosphorylation after translation.,

17Apart from atmospheric sulfur sources like H2S, carbonyl sulfide (COS), and SO2, mostly sulfur is taken up by dedicated sulfate transporters from the soil in the soluble SO42− ions. This dissolved sulfate is observed by SULTRs, a multigene family H+/sulfate co-transporter. These are high and low-affinity transporters and are distributed in different plants organelles like vacuole, plastid, and chloroplast. High-affinity sulfate transporters (SULTR1;1, SULTR1;2, and SULTR1;3) are most abundant in the epidermis and cortex of the root and facilitate the absorption of sulfate from the soil. Low-affinity sulfate transporter (SULTR2;1, SULTR2;2, SULTR3;5) are abundant in parenchymatic tissue adjacent to the xylem and phloem. Low-affinity transporters help in the epidermis and the cortex region and work synergistically with high-affinity transporters., Absorbed SO42− transported to vacuoles with the help of SULTR4;1 and SULTR4;2 as well as distributed to other parts of the plant. These transporters are also helpful in the remobilization of stored sulfate. It has been reported that SULTR1;3, SULTR2;1, SULTR2;2, and SULTR3;5 involved in the transportation of sulfate from root to shoot via xylem while, SULTR1;3, SULTR2;1, SULTR2;2, and SULTR3;5 involved in the transporting sulfate from root to mesophyll cells of the leaves. Further, SULTR4;1, and SULTR4;2 helps in transporting sulfate to vacuoles of shoots and leaves and SULTR3;1, SULTR3;2, SULTR3;3, and SULTR3;4 take part in sulfate transport to chloroplasts and further assimilate into other biomolecules. Atmospheric SO2 is absorbed by stomata and converted into SO32− and subsequently takes part in the sulfur reduction pathway in substomatal spaces. Likewise, atmospheric H2S, COS is also taken up by stomata. In leaf mesophyll cells, H2S is assimilated by O-acetyl-serine (thiol)lyase for the biosynthesis of cysteine. After absorption COS has converted into CO2 and H2S through carbonic anhydrase (CA)., A schematic representation of uptake, transport, assimilation, and storage of sulfate from different sources at the cellular level has been described in Figure 3.

18A very little information is available related to fungal sulfate transporters. Some notable fungal sulfate permeases that have been studied belong to Serendipita indica, Saccharomyces cerevisiae, Neurospora crassa, and Penicillium chrysogenum.,, Sulfate uptake is a highly regulated step and appears to occur in fungi and plants, via a family of related transporter proteins. It has been shown that enzymes ATP sulfurylase and APS kinase, catalyze the early steps of sulfate assimilation, and of the Aspergillus enzyme, cysteine synthase, which produces cysteine from O-acetylserine., Two genes viz., cys-13 (permease I) and cys14 (permease II) which encode the N. crassa sulfate transporters are of high affinity in nature., Sulfur sources, like sulfate or methionine, regulate both the genes at the transcriptional level. Under sulfur starvation, both genes have been shown to be highly expressed. Studies in A. nidulans have demonstrated that the sB gene is located on chromosome VI, whose transcriptional regulation relies on sulfur sources., Defective sB gene strains did not grow on sulfate as the sole sulfur source. However, they grew well on choline sulfate, which is taken up by a different permease. It has been reported the defective strains’ resistant ability to the toxic analogs of sulfate like selenate and chromate, in the condition of non-repressing methionine availability., In a methionine-supplemented medium, sulfate assimilation, as well as sulfate uptake, is robustly suppressed in S. cerevisiae, A. nidulans, and N. crassa. However, experiments on mutants compromised with methionine to cysteine conversion indicated that the mutants are regulatory effectors., This transcriptional regulation is dependent on the sulfur metabolite repression (SMR) system. Recently, it has been observed that high-affinity sulfate transporter of S. indica (SiSulT) uptake sulfate from media and transfer to the host plant. However, the same fungi’ mutant strain fails to uptake and transfer sulfate to the host plant. Further, it has been shown that S. indica SiSulT helped the colonized plant to grow healthy under sulfate-deprived conditions.

19Biotic and abiotic stresses adversely affect plant growth and crop productivity. However, nature has evolved several internal mechanisms to cope up with all these stresses. Sulfur plays an important role in different metabolic processes under the normal physiological condition as well as different stress conditions. Sulfur is the constituent of several compounds like amino acids (cysteine and methionine), vitamins (thiamine and biotin), coenzymes, thioredoxin system, glutathione, lipoic acids, and glucosinolates that directly or indirectly take part in ameliorating the adverse effects of different types of biotic and abiotic stresses. Moreover, sulfur-containing compounds also act as antioxidants that directly modulate the antioxidant defense system in order to save plants from biotic stresses. Sulfur-containing secondary compounds like sulfolipid and sulfoprotein take part in enzymatic steps related to oxidative stress. Sulfur-containing amino acids also interact with biomolecules like phytohormones, polyamines, nitric oxide (NO) in order to reduce abiotic stress. Studies suggest that sulfur derivatives accelerate signaling cascades to produce more cellular messengers like NO, Ca2+ and abscisic acid which ultimately helps in the initiation of other signaling networks related to stress tolerance.,, Soil sulfur is taken up by plant roots in a metabolically inert form sulfate (SO42-) and it is further processed for its assimilation. The enzyme ATP-sulfurylase (ATP-S) catalyzes the first committed step of sulfur assimilation. It covert SO42- into high-energy compound adenosine-5′-phosphosulfate (APS) and which is further reduced into sulfide (S2-) and incorporated into cysteine (Cys). Further, Cys helps in the synthesis of several sulfur-containing compounds like phytochelatins (PCs), methionine (Met), glutathione (GSH), and homo-GSH (h-GSH). Among them, PCs, GSH, and h-GSH are involved in different abiotic stress tolerance in the plants. Additionally, Met controls the very well-known ethylene signaling for tolerance against the different abiotic stresses through its first metabolite S-adenosylmethionine. The role, regulation, and underlying mechanisms of ATP-S in plant abiotic and biotic stress tolerance is also emphasized in several studies that indicate its intrinsic regulation by major sulfur-containing compounds.

20H2S is an emerging novel gaseous signaling molecule that takes part in several metabolic processes and signaling in plants. Studies show that H2S actively takes part in regulating several physiological processes like seed germination, maturation, senescence as well as overall plant growth and development. Additionally, it takes part in the plant defense system as well as the acquisition of stress tolerance. Being a gaseous compound, it can easily diffuse in different cellular parts and provide sulfur to the cells and counterbalance the antioxidant pools in cells as well. H2S signaling actively takes part in abiotic stresses and increases plant tolerance toward several adverse conditions by activating several associated other mechanisms like oxidative stress signaling, metal transport, Na+/K+ homeostasis, and antioxidative defense system. H2S also take part in crosstalk in different stress signaling through a complex signaling network that consists of several secondary metabolites and biomolecules such as NO, H2O2, Ca2+, and phytohormones.,, For instance, the H2S signaling initiated by abiotic stress leads to cross induction of signaling against several other stresses like drought, salinity, heavy metal, heat, cold, and flooding stress. This crosstalk signaling involved induction of several activities such as antioxidant activation, heat shock proteins production, accumulating osmolytes, and maintaining nutrient/ion balance. Further, during abiotic stress, H2S maintains the H2S-Cys-cycle which is followed by post-translational modifications of cysteine residues. At a higher concentration, H2S shows cytotoxicity, and at a lower concentration, it shows cell signaling. Therefore, it is very important to maintain H2S homeostasis to exert its physiological function as well as crosstalk signaling. Plants have evolved several metabolic mechanisms to maintain the H2S homeostasis like other signal molecules. H2S homeostasis is mainly regulated by enzymes like cysteine synthase, sulfite reductase, cyanoalanine synthase, L-cysteine desulfhydrase, and D-cysteine desulfhydrase. L-cysteine desulfhydrase, and D-cysteine desulfhydrase degrades L-/D-cysteine to produce H2S. Sulfite reductase convert sulfite to H2S. Cyanoalanine synthase catalyzes the H2S production from cysteine in the presence of HCN. The formation of cysteine is catalyzed by cysteine synthase in which O-acetyl-(thiol)-serinelyase, can incorporate H2S into O-acetyl-L-serine and its opposite reaction produces H2S. So L-cysteine desulfhydrase and D-cysteine desulfhydrase is mainly synthesizing H2S in response to different stresses,

21Sulfur nutrition is essential for the growth and development of plants. Sulfur deficiency leads to retarded growth and yield. Sulfate permeases of plants and plant-associated organisms (fungi and bacteria) play a crucial role in sulfur uptake from soil. Plants are able to take up sulfate from the soil over a wide range of concentrations through the use of high-affinity and low-affinity transporters., These sulfate transporters belong to the major facilitator superfamily (MFS) group of membrane transport proteins. As mentioned in soils with low sulfur availability, a symbiotic association between plants and an AMF assists with the sulfur acquisition from the soil: plants obtain nutrients from their fungal partner, which in return receives sugars from the plant., In this association, fungal and plant membrane transporters participate in nutrient transfer to the host plant. However, due to the lack of a stable transformation system in the case of AMF, the sulfate transporter system of AMF could not be manipulated to improve sulfur uptake in colonized plants. On the other hand, the beneficial endophyte S. indica can be cultivated axenically under laboratory conditions and has a well-established transformation system therefore functions of various genes have been studied.,, Colonization by S. indica improves a plant’s ability to acquire phosphorus, magnesium, iron, and sulfate from a nutrient-deprived soil rhizosphere,,,, due to the presence of dedicated nutrient transporters providing benefits to the host plant such as improved growth and increased resistance to biotic and abiotic stresses.,– Therefore, S. indica has been termed a plant probiotic. The versatile potential of S. indica makes it a promising agent in agricultural applications. Understanding the mechanism that S. indica utilizes to improve plant growth opens exciting avenues to further improve the fungal talents. In our opinion, despite all these novel approaches to improve sulfur enrichment in plants, detailed studies on the sulfate permeases and high-affinity sulfate transporters from the plant side as well as from associated partners are highly required to encourage sustainable agriculture and to reduce the load of chemical fertilizers. The crosstalk between plant and fungal/bacterial partners at the molecular level is less known. Hence, future studies on inter-organismic nutrient transfer can open new vistas to improve the nutrient exchange and hence plant growth and development. Sulfur and sulfur-containing compounds have been playing important role in the growth and development of plants. They play role in catalyzing several metabolic processes, as well as a cross mediator in different biotic and abiotic stress tolerance. Sulfur deficiency in the soil became the key factor limiting crop growth and yield. So far, as compared to other nutrients, studies on sulfur absorption, metabolism, regulation, and its mechanistic understanding is not enough and remain obscure. Thus, in the future, to improve the sulfur utilization efficiency in plants, more effort is required to know the regulatory mechanisms of plant’s response toward sulfur deficiency in soil and different stresses. The summary of this study is described in Figure 4.