Comparative Performance of Four Methods for High-throughput Glycosylation Analysis of Immunoglobulin G in Genetic and Epidemiological Research

Abstract

The biological and clinical relevance of glycosylation is becoming increasingly recognized, leading to a growing interest in large-scale clinical and population-based studies. In the past few years, several methods for high-throughput analysis of glycans have been developed, but thorough validation and standardization of these methods is required before significant resources are invested in large-scale studies. In this study, we compared liquid chromatography, capillary gel electrophoresis, and two MS methods for quantitative profiling of N-glycosylation of IgG in the same data set of 1201 individuals. To evaluate the accuracy of the four methods we then performed analysis of association with genetic polymorphisms and age. Chromatographic methods with either fluorescent or MS-detection yielded slightly stronger associations than MS-only and multiplexed capillary gel electrophoresis, but at the expense of lower levels of throughput. Advantages and disadvantages of each method were identified, which should inform the selection of the most appropriate method in future studies.

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Cited by papers we hold 6

• Community evaluation of glycoproteomics informatics solutions reveals high-performance search strategies for serum glycopeptide analysis · 2021
  Corrected
• MS-Based Allotype-Specific Analysis of Polyclonal IgG-Fc N-Glycosylation · 2020
  Not checked
• Glycosylation of immunoglobulin G is regulated by a large network of genes pleiotropic with inflammatory diseases · 2020
  No notice
• Association of Systemic Lupus Erythematosus With Decreased Immunosuppressive Potential of the IgG Glycome · 2015
  No notice
• Human plasma protein N-glycosylation · 2015
  No notice
• Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles—Part 1: Separation-based methods · 2015
  No notice

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