Advances in 3D single particle localization microscopy

The spatial resolution of conventional optical microscopy is limited by diffraction to transverse and axial resolutions of about 250 nm, but localization of point sources, such as single molecules or fluorescent beads, can be achieved with a precision of 10 nm or better in each direction. Traditional approaches to localization microscopy in two dimensions enable high precision only for a thin in-focus layer that is typically much less than the depth of a cell. This precludes, for example, super-resolution microscopy of extended three-dimensional biological structures or mapping of blood velocity throughout a useful depth of vasculature. Several techniques have been reported recently for localization microscopy in three dimensions over an extended depth range. We describe the principles of operation and typical applications of the most promising 3D localization microscopy techniques and provide a comparison of the attainable precision for each technique in terms of the Cramér-Rao lower bound for high-resolution imaging.

Abstract licence: CC BY

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