3D microvascular architecture of pre-cancerous lesions and invasive carcinomas of the colon

Abstract

Despite the significance of tumour neoangiogenesis and the extensive knowledge on the molecular basis of blood vessel formation currently no quantitative data exist on the 3D microvascular architecture in human primary tumours and their precursor lesions. This prompted us to examine the 3D vascular network of normal colon mucosa, adenomas and invasive carcinomas by means of quantitative microvascular corrosion casting. Fresh hemicolectomy specimens from 20 patients undergoing cancer or polyposis coli surgery were used for corrosion casting, factor VIII and VEGF immunostaining. In addition, immunostaining was done on colorectal tissue from 33 patients with metastatic and non-metastatic carcinomas, polyposis coli and adenomas. This first quantitative analysis of intervessel and interbranching distances, branching angles and vessel diameters in human cancer specimens revealed distinct patterns of the microvascular unit in the tumour centre and periphery. Irrespective of the tumour localization and grading all individual tumours displayed qualitatively and quantitatively the same vascular architecture. This gives further evidence for the existence of a tumour type-specific vascular architecture as recently demonstrated for experimental tumours. Metastatic tumours displayed different vascular architectures only within hot spots, in terms of smaller intervascular distances than in non-metastatic tumours. Pre-cancerous lesions have in part virtually the same vascular architecture like invasive carcinomas. Comparison of VEGF immunostaining also suggests that angiogenesis sets in long before the progress towards invasive phenotypes and that the so-called angiogenic switch is more likely a sequence of events.

References 26

1. 1.

   Ajiki T., Fujimori T., Ikehara H., Saitoh Y., Maeda S. K-ras gene mutation related to histological atypias in human colorectal adenomas. Biotech Histochem. 1995;70(2):90-94.

   Not in our library10.3109/10520299509108323

2. 2.

   Arbiser J. L., Moses M. A., Fernandez C. A., Ghiso N., Cao Y., Klauber N., Frank D., Brownlee M., Flynn E., Parangi S. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc Natl Acad Sci U S A. 1997;94(3):861-866.

   Not in our library10.1073/pnas.94.3.861

3. 3.

   Carmeliet P. Mechanisms of angiogenesis and arteriogenesis. Nat Med. 2000;6(4):389-395.

   Not in our library10.1038/74651

4. 4.

   Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease. Nat Med. 1995;1(1):27-31.

   Not in our library10.1038/nm0195-27

5. 5.

   Folkman J., D'Amore P. A. Blood vessel formation: what is its molecular basis? Cell. 1996;87(7):1153-1155.

   Not in our library10.1016/s0092-8674(00)81810-3

6. 6.

   Folkman J. The vascularization of tumors. Sci Am. 1976;234(5):58-73.

   Not in our library10.1038/scientificamerican0576-58

7. 7.

   Folkman J., Watson K., Ingber D., Hanahan D. Induction of angiogenesis during the transition from hyperplasia to neoplasia. Nature. 1989;339(6219):58-61.

   Not in our library10.1038/339058a0

8. 8.

   Herlyn M., Clark W. H., Rodeck U., Mancianti M. L., Jambrosic J., Koprowski H. Biology of tumor progression in human melanocytes. Lab Invest. 1987;56(5):461-474.

   Not in our library

9. 9.

   Ishikawa H., Fujii H., Yamamoto K., Morita T., Hata M., Koyama F., Terauchi S., Sugimori S., Kobayashi T., Enomoto H. Tumor angiogenesis predicts recurrence with normal serum carcinoembryonic antigen in advanced rectal carcinoma patients. Surg Today. 1999;29(10):983-991.

   Not in our library10.1007/s005950050633

10. 10.

    Jain R. K. Determinants of tumor blood flow: a review. Cancer Res. 1988;48(10):2641-2658.

    Not in our library

11. 11.

    Jakob W., Jentzsch K. D., Mauersberger B., Oehme P. Demonstration of angiogenesis-activity in the corpus luteum of cattle. Exp Pathol (Jena). 1977;13(4-5):231-236.

    Not in our library10.1016/s0014-4908(77)80007-0

12. 12.

    Kitadai Y., Haruma K., Tokutomi T., Tanaka S., Sumii K., Carvalho M., Kuwabara M., Yoshida K., Hirai T., Kajiyama G. Significance of vessel count and vascular endothelial growth factor in human esophageal carcinomas. Clin Cancer Res. 1998;4(9):2195-2200.

    Not in our library

13. 13.

    Konerding M. A., Fait E., Dimitropoulou C., Malkusch W., Ferri C., Giavazzi R., Coltrini D., Presta M. Impact of fibroblast growth factor-2 on tumor microvascular architecture. A tridimensional morphometric study. Am J Pathol. 1998;152(6):1607-1616.

    Not in our library

14. 14.
    Evidence for characteristic vascular patterns in solid tumours: quantitative studies using corrosion casts · 1999

    No notice
15. 15.

    Konerding M. A., Miodonski A. J., Lametschwandtner A. Microvascular corrosion casting in the study of tumor vascularity: a review. Scanning Microsc. 1995;9(4):1233-1244.

    Not in our library

16. 16.

    Malkusch W., Konerding M. A., Klapthor B., Bruch J. A simple and accurate method for 3-D measurements in microcorrosion casts illustrated with tumour vascularization. Anal Cell Pathol. 1995;9(1):69-81.

    Not in our library

17. 17.

    Millauer B., Shawver L. K., Plate K. H., Risau W., Ullrich A. Glioblastoma growth inhibited in vivo by a dominant-negative Flk-1 mutant. Nature. 1994;367(6463):576-579.

    Not in our library10.1038/367576a0

18. 18.

    Okada F., Rak J. W., Croix B. S., Lieubeau B., Kaya M., Roncari L., Shirasawa S., Sasazuki T., Kerbel R. S. Impact of oncogenes in tumor angiogenesis: mutant K-ras up-regulation of vascular endothelial growth factor/vascular permeability factor is necessary, but not sufficient for tumorigenicity of human colorectal carcinoma cells. Proc Natl Acad Sci U S A. 1998;95(7):3609-3614.

    Not in our library10.1073/pnas.95.7.3609

19. 19.

    Smith-McCune K. K., Weidner N. Demonstration and characterization of the angiogenic properties of cervical dysplasia. Cancer Res. 1994;54(3):800-804.

    Not in our library

20. 20.

    Takahashi Y., Bucana C. D., Cleary K. R., Ellis L. M. p53, vessel count, and vascular endothelial growth factor expression in human colon cancer. Int J Cancer. 1998;79(1):34-38.

    Not in our library10.1002/(sici)1097-0215(19980220)79:1<34::aid-ijc7>3.0.co;2-x

21. 21.

    Takahashi Y., Tucker S. L., Kitadai Y., Koura A. N., Bucana C. D., Cleary K. R., Ellis L. M. Vessel counts and expression of vascular endothelial growth factor as prognostic factors in node-negative colon cancer. Arch Surg. 1997;132(5):541-546.

    Not in our library10.1001/archsurg.1997.01430290087018

22. 22.

    Weidner N., Carroll P. R., Flax J., Blumenfeld W., Folkman J. Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma. Am J Pathol. 1993;143(2):401-409.

    Not in our library

23. 23.

    Weidner N., Folkman J., Pozza F., Bevilacqua P., Allred E. N., Moore D. H., Meli S., Gasparini G. Tumor angiogenesis: a new significant and independent prognostic indicator in early-stage breast carcinoma. J Natl Cancer Inst. 1992;84(24):1875-1887.

    Not in our library10.1093/jnci/84.24.1875

24. 24.

    Weidner N., Semple J. P., Welch W. R., Folkman J. Tumor angiogenesis and metastasis--correlation in invasive breast carcinoma. N Engl J Med. 1991;324(1):1-8.

    Not in our library10.1056/nejm199101033240101

25. 25.

    Wong M. P., Cheung N., Yuen S. T., Leung S. Y., Chung L. P. Vascular endothelial growth factor is up-regulated in the early pre-malignant stage of colorectal tumour progression. Int J Cancer. 1999;81(6):845-850.

    Not in our library10.1002/(sici)1097-0215(19990611)81:6<845::aid-ijc1>3.0.co;2-5

26. 26.

    Yancopoulos G. D., Klagsbrun M., Folkman J. Vasculogenesis, angiogenesis, and growth factors: ephrins enter the fray at the border. Cell. 1998;93(5):661-664.

    Not in our library10.1016/s0092-8674(00)81426-9

Cited by papers we hold 4

• Lactate and glutamine support NADPH generation in cancer cells under glucose deprived conditions · 2021
  Not checked
• A detailed comparison between the endoscopic images using blue laser imaging and three-dimensional reconstructed pathological images of colonic lesions. · 2020
  Not checked
• Narrow Band Imaging and Autofluorescence Imaging for the Detection and Optical Diagnosis of Colorectal Polyps · 2010
  Not checked
• Vascular disrupting agents in clinical development · 2007
  No notice

Reads

A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.