Engineering subtilisin proteases that specifically degrade active RAS
Yingwei Chen, Eric A. Toth, Biao Ruan and 16 more
Communications Biology · 2021
doi:10.1038/s42003-021-01818-7 (opens in a new tab)
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Abstract
Chen et al. describe a rational design of subtilisin mutants that degrade active RAS by cleaving a conserved sequence in switch 2. They further modified the active site to be dependent on a cofactor to generate high target specificity. Proteases engineered to cleave this region degraded RAS in vitro and in cells with a promise of adaptability for other target proteins too.
Abstract licence: CC BY-NC-ND
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