Identification of the potential mechanism of Radix pueraria in colon cancer based on network pharmacology

Radix Puerariae (RP), a dry root of Pueraria lobata (Willd.) Ohwi, is used to treat a variety of diseases, including cancer. Several in vitro and in vivo studies have demonstrated the efficacy of RP in the treatment of colon cancer (CC). However, the biological mechanism of RP in the treatment of colon cancer remains unclear. In this study, the active component of RP and its potential molecular mechanism against CC were studied by network pharmacology and enrichment analysis. The methods adopted included screening active ingredients of Chinese medicine, predicting target genes of Chinese medicine and disease, constructing of a protein interaction network, and conducting GO and KEGG enrichment analysis. Finally, the results of network pharmacology were further validated by molecular docking experiments and cell experiments. Eight active constituents and 14 potential protein targets were screened from RP, including EGFR, JAK2 and SRC. The biological mechanism of RP against CC was analysed by studying the relationship between active components, targets, and enrichment pathways. These findings provide a basis for understanding the clinical application of RP in CC.

Abstract licence: CC BY-NC-ND

1. 3.

   A Network Pharmacology Approach to Determine Active Compounds and Action Mechanisms of Ge‐Gen‐Qin‐Lian Decoction for Treatment of Type 2 Diabetes · 2014

2. 13.

   TCMSP: a database of systems pharmacology for drug discovery from herbal medicines · 2014

3. 18.

   OMIM.org: Online Mendelian Inheritance in Man (OMIM®), an online catalog of human genes and genetic disorders · 2014

4. 19.

   SwissTargetPrediction: a web server for target prediction of bioactive small molecules · 2014

5. 22.

   Epidermal Growth Factor Receptor Cell Proliferation Signaling Pathways · 2017

6. 25.

   Prediction and analysis of essential genes using the enrichments of gene ontology and KEGG pathways · 2017

7. 27.

   Emerging functions of the EGFR in cancer · 2017

8. 33.

   Metascape provides a biologist-oriented resource for the analysis of systems-level datasets · 2019

9. 37.

   Formononetin: A Review of Its Anticancer Potentials and Mechanisms · 2019

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