A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
Frederic Strobl, Ernst H. K. Stelzer
Scientific Reports · 2021
doi:10.1038/s41598-021-94288-0 (opens in a new tab)
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Abstract
Abstract We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings.
Abstract licence: CC BY-NC-ND
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