Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
Federico Colombo, Elisa Corsiero, Myles J. Lewis and 1 more
Scientific Reports · 2021
doi:10.1038/s41598-021-91264-6 (opens in a new tab)
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Abstract
Abstract Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting classical restriction site (RS) sequence in human SARS-CoV-2 genomes and (2) comparing them with other recombinant SARS-CoV-like virus created for experimental purposes. Finally, we propose a novel approach consisting in the generation of a restriction endonucleases site map of SARS-CoV-2 and other related coronavirus genomes to be used as a fingerprint to trace the virus evolution.
Abstract licence: CC BY-NC-ND
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