Genome-wide maps of nucleolus interactions reveal distinct layers of repressive chromatin domains
Cristiana Bersaglieri, Jelena Kresoja-Rakic, Shivani Gupta and 4 more
Nature Communications · 2022
doi:10.1038/s41467-022-29146-2 (opens in a new tab)
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Abstract
Mapping nucleolar-associated domains (NADs) is challenging as the nucleolus is membrane-less. Here the authors adapted the DNA adenine methyltransferase identification (DamID) method by engineering a “nucleolar histone” that can mark genomic contacts with the nucleolus through DNA methylation (m6A) and characterized NADs in mouse embryonic stem cells (ESCs) and derived neural progenitors (NPCs).
Abstract licence: CC BY-NC-ND
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