CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction
Jiao Liu, Moshi Liu, Tuo Shi and 13 more
Nature Communications · 2022
doi:10.1038/s41467-022-28501-7 (opens in a new tab)
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Abstract
Corynebacterium glutamicum is a major workhorse in industrial biomanufacturing of amino acids. Here, the authors employ CRISPR-assisted rational flux-tuning and CRISPRi screening of a L-proline exporter to covert a wild-type C. glutamicum to a hyperproducer of L-proline.
Abstract licence: CC BY-NC-ND
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