Capillary flow experiments for thermodynamic and kinetic characterization of protein liquid-liquid phase separation

Methods to quantitatively study liquid-liquid phase separation (LLPS) of proteins are lacking. Here the authors report Capillary flow experiments (Capflex) for the quantification of key LLPS parameters; they study Ddx4, the RP3 peptide and the aberrant liquid-to-solid phase transition of α-synuclein.

Abstract licence: CC BY-NC-ND

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