Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish
Stefan Hans, Daniela Zöller, Juliane Hammer and 9 more
Nature Communications · 2021
doi:10.1038/s41467-021-21427-6 (opens in a new tab)
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Abstract
Targeting a gene with two loxP sites is both time and labour intensive. Here the authors present Cre-Controlled CRISPR allowing conditional mutagenesis of a gene of interest and simultaneously labelling the putative mutant cells fluorescently.
Abstract licence: CC BY-NC-ND
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