Visualizing group II intron dynamics between the first and second steps of splicing
Jacopo Manigrasso, Isabel Chillón, Vito Genna and 5 more
Nature Communications · 2020
doi:10.1038/s41467-020-16741-4 (opens in a new tab)
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Abstract
Group II introns are self-splicing ribozymes. Here, the authors employ enzymatic assay, X-ray crystallography and molecular dynamics simulations to show that protonation of the group II intron catalytic triad plays an important role for the transition from the first to the second step of splicing.
Abstract licence: CC BY-NC-ND
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