Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition
Zhong Yao, Farzaneh Aboualizadeh, Jason Kroll and 8 more
Nature Communications · 2020
doi:10.1038/s41467-020-16299-1 (opens in a new tab)
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Abstract
Protein-protein interactions are fundamental to the regulation of protein activity and cellular phyisology. Here the authors present Split Intein-Mediated Protein Ligation, which uses bait and prey proteins fused to intein fragments to generate single intact proteins upon interaction.
Abstract licence: CC BY-NC-ND
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