Photoactivation of silicon rhodamines via a light-induced protonation
Michelle S. Frei, Philipp Hoess, Marko Lampe and 8 more
Nature Communications · 2019
doi:10.1038/s41467-019-12480-3 (opens in a new tab)
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Abstract
Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.
Abstract licence: CC BY-NC-ND
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