A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold
Yuxi Liu, Duc T. Huynh, Todd O. Yeates
Nature Communications · 2019
doi:10.1038/s41467-019-09836-0 (opens in a new tab)
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Abstract
Proteins smaller than about 50 kDa are currently too small to be imaged at high resolution by cryo‐electron microscopy (cryo‐EM). Here authors design a protein scaffold that binds 12 copies of a small 26 kDa protein (GFP), which allowed visualizing GFP at a resolution of 3.8Å by cryo‐EM.
Abstract licence: CC BY-NC-ND
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