Interactions between horizontally acquired genes create a fitness cost in Pseudomonas aeruginosa

Abstract

Horizontal gene transfer (HGT) plays a key role in bacterial evolution, especially with respect to antibiotic resistance. Fitness costs associated with mobile genetic elements (MGEs) are thought to constrain HGT, but our understanding of these costs remains fragmentary, making it difficult to predict the success of HGT events. Here we use the interaction between P. aeruginosa and a costly plasmid (pNUK73) to investigate the molecular basis of the cost of HGT. Using RNA-Seq, we show that the acquisition of pNUK73 results in a profound alteration of the transcriptional profile of chromosomal genes. Mutations that inactivate two genes encoded on chromosomally integrated MGEs recover these fitness costs and transcriptional changes by decreasing the expression of the pNUK73 replication gene. Our study demonstrates that interactions between MGEs can compromise bacterial fitness via altered gene expression, and we argue that conflicts between mobile elements impose a general constraint on evolution by HGT.

References 53

1. 1.

   This reference’s wording can’t be shown under the paper’s licence.

   Not in our library10.1038/35012500

2. 2.

   This reference’s wording can’t be shown under the paper’s licence.

   Not in our library10.1111/j.1574-6976.2011.00292.x

3. 3.

   This reference’s wording can’t be shown under the paper’s licence.

   Not in our library10.1016/j.ijmm.2013.02.001

4. 4.

   This reference’s wording can’t be shown under the paper’s licence.

   Not in our library10.1016/j.cell.2007.03.004

5. 5.

   This reference’s wording can’t be shown under the paper’s licence.

   Not in our library10.1016/j.tree.2013.04.002

6. 6.
   The genetic basis of the fitness costs of antimicrobial resistance: a meta‐analysis approach · 2014

   No notice
7. 7.

   This reference’s wording can’t be shown under the paper’s licence.

   Not in our library10.3389/fcimb.2014.00054

8. 8.

   This reference’s wording can’t be shown under the paper’s licence.

   Not in our library10.1111/j.1365-2958.2004.04113.x

9. 9.

   This reference’s wording can’t be shown under the paper’s licence.

   Not in our library10.1016/j.tig.2008.10.007

10. 10.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1016/j.molcel.2010.04.015

11. 11.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1093/bioinformatics/bti1136

12. 12.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1073/pnas.83.15.5723

13. 13.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1002/biot.201100062

14. 14.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1073/pnas.1017570108

15. 15.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1093/gbe/evs030

16. 16.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1093/molbev/msq333

17. 17.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1186/gb-2007-8-8-r156

18. 18.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1126/science.1128794

19. 19.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1126/science.1137550

20. 20.
    Positive selection and compensatory adaptation interact to stabilize non-transmissible plasmids · 2014

    No notice
21. 21.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1007/s00253-002-1195-1

22. 22.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1046/j.1365-2958.2001.02661.x

23. 23.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1128/jb.00807-06

24. 24.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1038/nprot.2006.24

25. 25.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1111/j.1574-6968.1999.tb08811.x

26. 26.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1111/j.1365-2958.2007.06051.x

27. 27.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1128/jb.175.4.1118-1125.1993

28. 28.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1128/jb.01515-09

29. 29.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1038/hdy.2010.24

30. 30.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1128/jb.187.14.4728-4738.2005

31. 31.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1128/aem.05439-11

32. 32.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1128/aac.00783-09

33. 33.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1128/jb.185.7.2066-2079.2003

34. 34.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1093/nar/gkt942

35. 35.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1073/pnas.0801269105

36. 36.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1128/mmbr.62.2.434-464.1998

37. 37.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1038/sj.embor.embor703

38. 38.
    Pervasive sign epistasis between conjugative plasmids and drug-resistance chromosomal mutations. · 2011

    Not checked
39. 39.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1038/ismej.2013.182

40. 40.

    This reference’s wording can’t be shown under the paper’s licence.

    Not in our library10.1073/pnas.1204615109

Cited by papers we hold 6

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• Variability of plasmid fitness effects contributes to plasmid persistence in bacterial communities · 2021
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• CRISPR-Cas systems restrict horizontal gene transfer in Pseudomonas aeruginosa · 2020
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• Emergence of plasmid stability under non-selective conditions maintains antibiotic resistance · 2019
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• Persistence and reversal of plasmid-mediated antibiotic resistance · 2017
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• Horizontal Transfer of the <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Infantis Resistance and Virulence Plasmid pESI to the Gut Microbiota of Warm-Blooded Hosts · 2016
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