Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour

Abstract

Previous efforts to control cellular behaviour have largely relied upon various forms of genetic engineering. Once the genetic content of a living cell is modified, the behaviour of that cell typically changes as well. However, other methods of cellular control are possible. All cells sense and respond to their environment. Therefore, artificial, non-living cellular mimics could be engineered to activate or repress already existing natural sensory pathways of living cells through chemical communication. Here we describe the construction of such a system. The artificial cells expand the senses of Escherichia coli by translating a chemical message that E. coli cannot sense on its own to a molecule that activates a natural cellular response. This methodology could open new opportunities in engineering cellular behaviour without exploiting genetically modified organisms.

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Cited by papers we hold 7

• On the “Life-Likeness” of Synthetic Cells · 2020
  Not checked
• Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology · 2020
  Not checked
• Intercellular communication between artificial cells by allosteric amplification of a molecular signal · 2020
  No notice
• Artificial cells drive neural differentiation · 2020
  No notice
• The hallmarks of living systems: towards creating artificial cells · 2018
  No notice
• Current Ideas about Prebiological Compartmentalization · 2015
  No notice
• Synthetic Biology Outside the Cell: Linking Computational Tools to Cell-Free Systems · 2014
  Not checked

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