Structure and assembly of scalable porous protein cages

Self-assembling proteins that form capsid-like structures act as molecular containers for diverse cargoes. Here, the authors solve the cryo-EM structures of lumazine synthase shells, and show that supercharged mutants form expanded assemblies, indicating that electrostatics can be exploited to engineer cage architecture.

Abstract licence: CC BY-NC-ND

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   RELION: Implementation of a Bayesian approach to cryo-EM structure determination · 2012

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