4Pi-RESOLFT nanoscopy
Ulrike Böhm, Stefan W. Hell, Roman Schmidt
Nature Communications · 2016
doi:10.1038/ncomms10504 (opens in a new tab)
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Abstract
The ability to discriminate objects along the optic axis is a benchmark for three-dimensional imaging techniques. Here, the authors combine metastable-state switching and opposing objective lenses to suppress out-of-focus background and record three-dimensional nanoscale images of living cells.
Abstract licence: CC BY-NC-ND
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