Evaluation of epidermal growth factor receptors in bladder tumours

Epidermal growth factor (EGF) receptor expression in 31 primary human bladder tumours was quantitated using both structural and functional assays and the EGF receptor gene in the same tumours was analyzed by Southern blot analysis. Immunocytochemical studies using the EGFR1 monoclonal antibody (Mab) showed a significant correlation between EGF receptor levels and the stage and grade of the tumours. Autophosphorylation assays employed to evaluate the receptor's tyrosine kinase activity gave results which in general were consistent with the immunocytochemical data. Using internally controlled immunocytochemical studies with two Mabs and Southern blot analysis of DNA isolated from the tumours, no evidence was obtained for the production of truncated receptors similar to those encoded by the v-erb-B oncogene. Gene amplification was not found in any of the superficial tumours, but one invasive tumour with high EGF receptor expression had an 8-10 fold amplification of the EGF receptor gene. The EGF receptor isolated from this tumour showed a normal pattern of tyrosine phosphorylation at all three major autophosphorylation sites. Our detailed study is consistent with the correlation previously found between EGF receptor expression and stage and grade of bladder tumours, and suggests that at this level of analysis EGF receptors in bladder tumours are not abnormal in structure or size, autophosphorylation activity, or gene structure.

Abstract licence: CC BY

1. 1.

   Bargmann C. I., Hung M. C., Weinberg R. A. Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Cell. 1986;45(5):649-657.

   Not in our library10.1016/0092-8674(86)90779-8 (opens in a new tab)

2. 2.

   Brodeur G. M., Seeger R. C., Sather H., Dalton A., Siegel S. E., Wong K. Y., Hammond D. Clinical implications of oncogene activation in human neuroblastomas. Cancer. 1986;58(2 Suppl):541-545.

   Not in our library10.1002/1097-0142(19860715)58:2+<541::aid-cncr2820581322>3.0.co;2-2 (opens in a new tab)

3. 3.

   Carpenter G. The biochemistry and physiology of the receptor-kinase for epidermal growth factor. Mol Cell Endocrinol. 1983;31(1):1-19.

   Not in our library10.1016/0303-7207(83)90027-8 (opens in a new tab)

4. 4.

   Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984;32(2):219-229.

   Not in our library10.1177/32.2.6198355 (opens in a new tab)

5. 5.

   Coussens L., Van Beveren C., Smith D., Chen E., Mitchell R. L., Isacke C. M., Verma I. M., Ullrich A. Structural alteration of viral homologue of receptor proto-oncogene fms at carboxyl terminus. Nature. 1986;320(6059):277-280.

   Not in our library10.1038/320277a0 (opens in a new tab)

6. 6.

   Downward J., Parker P., Waterfield M. D. Autophosphorylation sites on the epidermal growth factor receptor. Nature. 1984;311(5985):483-485.

   Not in our library10.1038/311483a0 (opens in a new tab)

7. 7.

   Downward J., Yarden Y., Mayes E., Scrace G., Totty N., Stockwell P., Ullrich A., Schlessinger J., Waterfield M. D. Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences. Nature. 1984;307(5951):521-527.

   Not in our library10.1038/307521a0 (opens in a new tab)

8. 8.

   Filmus J., Pollak M. N., Cailleau R., Buick R. N. MDA-468, a human breast cancer cell line with a high number of epidermal growth factor (EGF) receptors, has an amplified EGF receptor gene and is growth inhibited by EGF. Biochem Biophys Res Commun. 1985;128(2):898-905.

   Not in our library10.1016/0006-291x(85)90131-7 (opens in a new tab)

9. 9.

   Fitzpatrick S. L., Brightwell J., Wittliff J. L., Barrows G. H., Schultz G. S. Epidermal growth factor binding by breast tumor biopsies and relationship to estrogen receptor and progestin receptor levels. Cancer Res. 1984;44(8):3448-3453.

   Not in our library

10. 10.

    Fujita J., Yoshida O., Yuasa Y., Rhim J. S., Hatanaka M., Aaronson S. A. Ha-ras oncogenes are activated by somatic alterations in human urinary tract tumours. Nature. 309(5967);464-466.

    Not in our library10.1038/309464a0 (opens in a new tab)

11. 11.

    Gilmore T., DeClue J. E., Martin G. S. Protein phosphorylation at tyrosine is induced by the v-erbB gene product in vivo and in vitro. Cell. 1985;40(3):609-618.

    Not in our library10.1016/0092-8674(85)90209-0 (opens in a new tab)

12. 12.

    Gullick W. J., Downward J., Waterfield M. D. Antibodies to the autophosphorylation sites of the epidermal growth factor receptor protein-tyrosine kinase as probes of structure and function. EMBO J. 1985;4(11):2869-2877.

    Not in our library10.1002/j.1460-2075.1985.tb04016.x (opens in a new tab)

13. 13.

    Gullick W. J., Marsden J. J., Whittle N., Ward B., Bobrow L., Waterfield M. D. Expression of epidermal growth factor receptors on human cervical, ovarian, and vulval carcinomas. Cancer Res. 1986;46(1):285-292.

    Not in our library

14. 14.

    Gusterson B., Cowley G., McIlhinney J., Ozanne B., Fisher C., Reeves B. Evidence for increased epidermal growth factor receptors in human sarcomas. Int J Cancer. 1985;36(6):689-693.

    Not in our library10.1002/ijc.2910360612 (opens in a new tab)

15. 15.

    Gusterson B., Cowley G., Smith J. A., Ozanne B. Cellular localisation of human epidermal growth factor receptor. Cell Biol Int Rep. 1984;8(8):649-658.

    Not in our library10.1016/0309-1651(84)90045-6 (opens in a new tab)

16. 16.

    Hayashi K., Kakizoe T., Sugimura T. In vivo amplification and rearrangement of the c-Ha-ras-1 sequence in a human bladder carcinoma. Gan. 1983;74(6):798-801.

    Not in our library

17. 17.

    Hendler F. J., Ozanne B. W. Human squamous cell lung cancers express increased epidermal growth factor receptors. J Clin Invest. 1984;74(2):647-651.

    Not in our library10.1172/jci111463 (opens in a new tab)

18. 18.

    Hunts J., Ueda M., Ozawa S., Abe O., Pastan I., Shimizu N. Hyperproduction and gene amplification of the epidermal growth factor receptor in squamous cell carcinomas. Jpn J Cancer Res. 1985;76(8):663-666.

    Not in our library

19. 19.

    Kris R. M., Lax I., Gullick W., Waterfield M. D., Ullrich A., Fridkin M., Schlessinger J. Antibodies against a synthetic peptide as a probe for the kinase activity of the avian EGF receptor and v-erbB protein. Cell. 1985;40(3):619-625.

    Not in our library10.1016/0092-8674(85)90210-7 (opens in a new tab)

20. 20.

    Libermann T. A., Nusbaum H. R., Razon N., Kris R., Lax I., Soreq H., Whittle N., Waterfield M. D., Ullrich A., Schlessinger J. Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin. Nature. 1985;313(5998):144-147.

    Not in our library10.1038/313144a0 (opens in a new tab)

21. 21.

    Libermann T. A., Razon N., Bartal A. D., Yarden Y., Schlessinger J., Soreq H. Expression of epidermal growth factor receptors in human brain tumors. Cancer Res. 1984;44(2):753-760.

    Not in our library

22. 22.

    Malone P. R., Visvanathan K. V., Ponder B. A., Shearer R. J., Summerhayes I. C. Oncogenes and bladder cancer. Br J Urol. 1985;57(6):664-667.

    Not in our library10.1111/j.1464-410x.1985.tb07028.x (opens in a new tab)

23. 23.

    Mattila A. L., Pasternack A., Viinikka L., Perheentupa J. Subnormal concentrations of urinary epidermal growth factor in patients with kidney disease. J Clin Endocrinol Metab. 1986;62(6):1180-1183.

    Not in our library10.1210/jcem-62-6-1180 (opens in a new tab)

24. 24.

    Merlino G. T., Xu Y. H., Ishii S., Clark A. J., Semba K., Toyoshima K., Yamamoto T., Pastan I. Amplification and enhanced expression of the epidermal growth factor receptor gene in A431 human carcinoma cells. Science. 1984;224(4647):417-419.

    Not in our library10.1126/science.6200934 (opens in a new tab)

25. 25.

    Merlino G. T., Xu Y. H., Richert N., Clark A. J., Ishii S., Banks-Schlegel S., Pastan I. Elevated epidermal growth factor receptor gene copy number and expression in a squamous carcinoma cell line. J Clin Invest. 1985;75(3):1077-1079.

    Not in our library10.1172/jci111770 (opens in a new tab)

26. 26.

    Neal D. E., Marsh C., Bennett M. K., Abel P. D., Hall R. R., Sainsbury J. R., Harris A. L. Epidermal-growth-factor receptors in human bladder cancer: comparison of invasive and superficial tumours. Lancet. 1985;1(8425):366-368.

    Not in our library10.1016/s0140-6736(85)91386-8 (opens in a new tab)

27. 27.

    Oka Y., Orth D. N. Human plasma epidermal growth factor/beta-urogastrone is associated with blood platelets. J Clin Invest. 1983;72(1):249-259.

    Not in our library10.1172/jci110964 (opens in a new tab)

28. 28.

    Olofsson B., Pizon V., Zahraoui A., Tavitian A., Therwath A. Structure and expression of the chicken epidermal growth factor receptor gene locus. Eur J Biochem. 1986;160(2):261-266.

    Not in our library10.1111/j.1432-1033.1986.tb09965.x (opens in a new tab)

29. 29.

    Ozanne B., Richards C. S., Hendler F., Burns D., Gusterson B. Over-expression of the EGF receptor is a hallmark of squamous cell carcinomas. J Pathol. 1986;149(1):9-14.

    Not in our library10.1002/path.1711490104 (opens in a new tab)

30. 30.

    Pérez R., Pascual M., Macías A., Lage A. Epidermal growth factor receptors in human breast cancer. Breast Cancer Res Treat. 1984;4(3):189-193.

    Not in our library10.1007/bf01806484 (opens in a new tab)

31. 31.

    Riedel H., Schlessinger J., Ullrich A. A chimeric, ligand-binding v-erbB/EGF receptor retains transforming potential. Science. 1987;236(4798):197-200.

    Not in our library10.1126/science.3494307 (opens in a new tab)

32. 32.

    Sainsbury J. R., Farndon J. R., Sherbet G. V., Harris A. L. Epidermal-growth-factor receptors and oestrogen receptors in human breast cancer. Lancet. 1985;1(8425):364-366.

    Not in our library10.1016/s0140-6736(85)91385-6 (opens in a new tab)

33. 33.

    Seeger R. C., Brodeur G. M., Sather H., Dalton A., Siegel S. E., Wong K. Y., Hammond D. Association of multiple copies of the N-myc oncogene with rapid progression of neuroblastomas. N Engl J Med. 1985;313(18):1111-1116.

    Not in our library10.1056/nejm198510313131802 (opens in a new tab)

34. 34.

    Slamon D. J., Clark G. M., Wong S. G., Levin W. J., Ullrich A., McGuire W. L. Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science. 1987;235(4785):177-182.

    Not in our library10.1126/science.3798106 (opens in a new tab)

35. 35.

    Sukumar S., Notario V., Martin-Zanca D., Barbacid M. Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations. Nature. 1983;306(5944):658-661.

    Not in our library10.1038/306658a0 (opens in a new tab)

36. 36.

    Takeya T., Hanafusa H. Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virus. Cell. 1983;32(3):881-890.

    Not in our library10.1016/0092-8674(83)90073-9 (opens in a new tab)

37. 37.

    Viola M. V., Fromowitz F., Oravez S., Deb S., Finkel G., Lundy J., Hand P., Thor A., Schlom J. Expression of ras oncogene p21 in prostate cancer. N Engl J Med. 1986;314(3):133-137.

    Not in our library10.1056/nejm198601163140301 (opens in a new tab)

38. 38.

    Waterfield M. D., Mayes E. L., Stroobant P., Bennet P. L., Young S., Goodfellow P. N., Banting G. S., Ozanne B. A monoclonal antibody to the human epidermal growth factor receptor. J Cell Biochem. 1982;20(2):149-161.

    Not in our library10.1002/jcb.240200207 (opens in a new tab)

39. 39.

    Yamamoto T., Kamata N., Kawano H., Shimizu S., Kuroki T., Toyoshima K., Rikimaru K., Nomura N., Ishizaki R., Pastan I. High incidence of amplification of the epidermal growth factor receptor gene in human squamous carcinoma cell lines. Cancer Res. 1986;46(1):414-416.

    Not in our library

• Amplification and over-expression of c-erbB-2 in transitional cell carcinoma of the urinary bladder · 1991

• Gene amplification and overexpression of EGF receptor in squamous cell carcinomas of the head and neck · 1989

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