A fast atom bombardment-mass spectrometric method to quantitate lysophosphatidylserine in rat brain.
E Benfenati, G De Bellis, S Chen and 5 more
Journal of Lipid Research · 1989
doi:10.1016/s0022-2275(20)38210-9 (opens in a new tab)
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Abstract
A method to quantitate lysophosphatidylserine by fast atom bombardment-mass spectrometry using 1-hexa-decanoyl-sn-glycero-3-phospho-L-serine as internal standard is described. The standard curve is linear with a correlation coefficient r2 = 0.999 from 10 to 1000 ng. This curve has been used to quantitate LPS in rat brain using phosphorus assay as a test control. We found 475 +/- 70 ng of LPS in 1 mg of tissue (n = 3). This method presents advantages due to its sensitivity and its capability to give molecular information of the unmodified compound.
Abstract licence: CC BY-NC-ND
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