New sensitive assay for phosphatidylserine decarboxylase based on the detection of CO2 from nonradiolabeled phosphatidylserine

A new rapid assay for phosphatidylserine decarboxylase, which is sensitive in the nanomolar range, is described. Synthesis of radiolabeled phosphatidylserine for use as a substrate is not required, since the assay, unlike previous ones, is based on the detection of CO2 liberated from unlabeled phosphatidylserine. The assay employs a gas chromatographic procedure for the analysis of methane formed by catalytic conversion of the C02 produced as a product of the enzymatic reaction.

Abstract licence: CC BY-NC-ND

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