Protocol for electrical modulation of ERK signaling dynamics in cell lines
Kan Zhu, Liang Guo, Preena Patel and 4 more
STAR Protocols · 2022
doi:10.1016/j.xpro.2022.101752 (opens in a new tab)
We email you when a new notice or a question about this paper appears.
Abstract
Summary: Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity in real time without chemical agents or gene disruption. ERK activation is assessed by comparing the relative intensity of nuclear fluorescence to cytosolic fluorescence in live-cell conditions. The approach can be applied to other signaling pathways as well.For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).: Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Abstract licence: CC BY-NC
References4
4 more references, whose wording the paper’s licence keeps private.
Reads
Read by people on this page
None yet.
Checked or read by AI through naulon
None yet.
A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.
Are you an author?
Is this your paper?
Claim it to add your note and be paid when agents cite it. Free.