Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices
Juan Zhang, Wentao Ha, Yuhan Chang and 4 more
STAR Protocols · 2022
doi:10.1016/j.xpro.2022.101577 (opens in a new tab)
We email you when a new notice or a question about this paper appears.
Abstract
Summary: Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tumorigenic cell selection. We also describe how it can be applied to tumor mechanotransductive research by colony growth monitoring and cell isolation.For complete details on the use and execution of this protocol, please refer to Chang et al. (2022).: Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Abstract licence: CC BY-NC
Reads
Read by people on this page
None yet.
Checked or read by AI through naulon
None yet.
A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.
Are you an author?
Is this your paper?
Claim it to add your note and be paid when agents cite it. Free.