Protocol for studying microbiome impact on host energy and reproduction in Drosophila
Yulia Gnainsky, Maxim Itkin, Tevie Mehlman and 3 more
STAR Protocols · 2022
doi:10.1016/j.xpro.2022.101253 (opens in a new tab)
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Abstract
Summary: Drosophila gut microbiome in flies has been shown to have a systemic influence on energy production by the host and the energetic investment in growth and reproduction. Here we describe a protocol for studying the mechanisms responsible for this remote regulation by gut bacteria. This protocol enables whole-body and ovary-specific quantification of energy-storing molecules as well as identification of host metabolites and pathways that are regulated by gut microbiome-derived factors. Similar procedures are applicable to additional treatments and genetic manipulations.For complete details on the use and execution of this protocol, please refer to Gnainsky et al. (2021).
Abstract licence: CC BY-NC
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