Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing
Shanshan Cheng, Yuqian Shen, Zi Chao Zhang and 1 more
STAR Protocols · 2021
doi:10.1016/j.xpro.2021.100679 (opens in a new tab)
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Abstract
Summary: Metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) occurs in diverse brain regions and contributes to the plasticity of behavior, learning, and memory. mGluR-LTD relies on rapid (in minutes) local protein synthesis. Here, we describe a detailed protocol for delivering an interfering peptide into the adult mouse hippocampus. The delivered peptide disrupts the interaction between polyglutamine binding protein 1 and eukaryotic elongation factor 2, resulting in impaired hippocampal mGluR-LTD and mGluR-LTD-associated behaviors.For complete details on the use and execution of this protocol, please refer to Shen et al. (2021).
Abstract licence: CC BY-NC-ND
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