Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions
Hideaki Nishihara, Benjamin D. Gastfriend, Pelin Kasap and 3 more
STAR Protocols · 2021
doi:10.1016/j.xpro.2021.100563 (opens in a new tab)
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Abstract
Summary: We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusion barrier characteristics. A mature immune phenotype with proper endothelial adhesion molecule expression makes this model distinct from any other hPSC-derived in vitro blood-brain barrier (BBB) model and suitable to study immune cell migration across the BBB in a disease relevant and personalized fashion.For complete details on the use and execution of this protocol, please refer to Lian et al. (2014), Nishihara et al. (2020a).
Abstract licence: CC BY-NC
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