Generation of RAB39B knockout isogenic human embryonic stem cell lines to model RAB39B-mediated Parkinson's disease
Yujing Gao, Gabrielle R. Wilson, Kiymet Bozaoglu and 4 more
Stem Cell Research · 2018
doi:10.1016/j.scr.2018.02.015 (opens in a new tab)
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Abstract
Mutations in RAB39B are a known cause of X-linked early onset Parkinson's disease. Isogenic human embryonic stem cell lines carrying two independent deletions of RAB39B were generated using CRISPR/Cas9 genome editing tool. The deletions were confirmed by PCR and direct sequence analysis in two edited stem cell lines. Both cell lines showed pluripotency and displayed a normal karyotype. Further, they were able to form embryoid bodies in vitro, and express markers indicative of differentiation to the three germ layers.
Abstract licence: CC BY-NC
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