Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9
So-Jung Kim, Omer Habib, Jin-Soo Kim and 3 more
Stem Cell Research · 2017
doi:10.1016/j.scr.2016.12.027 (opens in a new tab)
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Abstract
Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human embryonic stem cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Cas9 genome editing method. The Nrf2 homozygous knockout H9 cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype.
Abstract licence: CC BY-NC
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