Characterization and in vivo biocompatibility analysis of synthetic hydroxyapatite compounds associated with magnetite nanoparticles for a drug delivery system in osteomyelitis treatment

This work aimed to characterize six compounds formulated with hydroxyapatite (HAP), magnetite nanoparticles (Fe3O4) and ciprofloxacin, using scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS), in addition to describing their in vivo biocompatibility and biodegradation processes, aiming their subsequent use for treatment and bone regeneration in osteomyelitis cases. Six New Zealand white rabbits were used for the biocompatibility study, in which four samples of the same compound were surgically implanted in each rabbit, between the fascia and muscle tissue. After 15, 30, 60 and 90 days, biopsies of one sample from each animal were performed, comprising the implants and adjacent tissues. The analyzed compounds are biocompatible and biodegradable, with an absorption time of more than 90 days. In addition, according to the results obtained by SEM, they have a complex microtopographical surface and structural and morphological characteristics required to its use as bone implants. In three compounds, EDS analysis showed homogenous relative proportions of Ca:Fe throughout their surface, and at the other three the Ca:Fe ratios were not shown to be homogenous at different points of the same compound. Based on the obtained results, the subsequent use of these materials for the evaluation of bone regeneration in infection cases is indicated.

Abstract licence: CC BY-NC-ND

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