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Practical application of stereological kidney methods in experimental animal models

María Teresa Fernández García, Paula Núñez Martínez, Vanessa García de la Fuente and 5 more

Nefrología (English Edition) · 2017

doi:10.1016/j.nefroe.2017.01.012 (opens in a new tab)

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Abstract

The kidneys are vital organs responsible for excretion, fluid and electrolyte balance and hormone production. The nephrons are the kidney's functional and structural units. The number, size and distribution of the nephron components contain relevant information on renal function. Stereology is a branch of morphometry that applies mathematical principles to obtain three-dimensional information from serial, parallel and equidistant two-dimensional microscopic sections. Because of the complexity of stereological studies and the lack of scientific literature on the subject, the aim of this paper is to clearly explain, through animal models, the basic concepts of stereology and how to calculate the main kidney stereological parameters that can be applied in future experimental studies.

Abstract licence: CC BY-NC-ND

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