Corrigendum: High-throughput, nonperturbing quantification of lipid droplets with digital holographic microscopy

High-throughput, nonperturbing quantification of lipid droplets with digital holographic microscopyJournal of Lipid ResearchVol. 59Issue 7PreviewIn vitro differentiating adipocytes are sensitive to liquid manipulations and have the tendency to float. Assessing adipocyte differentiation using current microscopy techniques involves cell staining and washing, while using flow cytometry involves cell retrieval in suspension. These methods induce biases, are difficult to reproduce, and involve tedious optimizations. In this study, we present digital holographic microscopy (DHM) as a label-free, nonperturbing means to quantify lipid droplets in differentiating adipocytes in a robust medium- to high-throughput manner. Full-Text PDF Open Access 59 (2018) PAGES 1301–1310 In the original article, there was an error in the Material and Methods, p.1302, section “Cell culture and adipocytic differentiation“. The dexamethasone concentration was stated 10 μM while it should be 1 μM. The correct sentence should read as follows: “Adipocytic differentiation was performed during 6 days using a differentiation cocktail containing dexamethasone (Sigma, catalog no. D4902; 1 μM), insulin (Sigma, catalog no. I0516; 5 μg/ml), and isobutylmethylxanthine (I5879, 0.5 mM).” The authors state that the correction does not affect the results and conclusions of the paper.

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