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FT-IR profiling reveals differential response of roots and leaves to salt stress in a halophyte Sesuvium portulacastrum (L.) L.

G.C. Nikalje, Jitendra Kumar, T.D. Nikam and 1 more

Biotechnology Reports · 2019

doi:10.1016/j.btre.2019.e00352 (opens in a new tab)

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Abstract

In a halophyte, Sesuvium portulacastrum (L.) L., we have applied Fourier Transform InfraRed (FT-IR) spectroscopy to detect the corresponding changes associated with salt-induced physiological changes under long- term treatment with 0, 100 and 500 mM NaCl. FT-IR profiles showed changes in chemical composition and functional groups of proteins, lipids and carbohydrates due to salt treatments, evident as differential FT-IR profiles in both roots and leaves specific to these metabolites. Further, the Principle Component Analysis (PCA) was applied to identify the main sources of variation in FT-IR data due to differential treatment. In PCA, the PC1 showed 85.55% and PC2 showed 18.18% variability in data and confirmed differential response of root and leaves to salt treatment in Sesuvium. The results suggest that FT-IR spectrometry can be used to study stress-induced metabolic changes in plants in relation to their salt tolerance. Keywords: Fourier Transform Infrared Spectroscopy, Sesuvium portulacastrum, Salt stress, Metabolic changes

Abstract licence: CC BY-NC

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