Identification and characterization of high-mobility group box 1 (HMGB1) from Nile tilapia (Oreochromis niloticus)

High-mobility group box1 (HMGB1) is a non-histone chromosome protein that not only participates in nuclear formation and transcriptional regulation of, but also acts as a pro-inflammatory factor. In this study, a HMGB1 gene (OnHMGB1) was identified and characterized from Nile tilapia (Oreochromis niloticus). The open reading frame of OnHMGB1 sequence was 621 bp, encoding a 207 amino acids peptide. The deduced OnHMGB1 protein contained two HMG boxes and an acidic tail, which shared high identities to other reported HMGB1. OnHMGB1 was widely distributed in many tissues of healthy tilapia and was significantly up-regulated in spleen and head kidney after Streptococcus agalactiae poly I:C stimulation in vivo. Moreover, the recombinant protein of OnHMGB1 (rOnHMGB1) could bind to DNA in a dose-dependent manner, promote inflammatory factor expression and bactericidal activity of monocytes/macrophages in vitro as well. Taken together, these results indicate that OnHMGB1 plays roles in immune response of Nile tilapia against pathogen infection.

Abstract licence: CC BY-NC

A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.